MicroRNA Expression Patterns of the Kidney in Hyperuricemia Mice Treated with Xiezhuo Chubi Decoction（泄浊除痹方）

• Published in: Chinese journal of integrative medicine Vol. 17; no. 1; pp. 35 - 42
• Main Author: 孙维峰 张娴娴 孙奋勇 徐伟 梁静 冯书梅 王天
• Format: Journal Article
• Language: English
• Published: 2011
• Subjects: microRNA; 小分子RNA; 尿酸血症; 昆明小鼠; 浓度检测; 表达模式
• ISSN: 1672-0415; 1993-0402

Objective：To investigate the effects of Xiezhuo Chubi Decoction（泄浊除痹方,XZCBD） on the microRNA expression patterns of kidney in mice with hyperuricemia.Methods：Sixty Kunming male mice were randomly divided into the high-,medium-,and low-dose XZCBD groups,benzbromarone group,model group, and control group.Except the control group,all mice were established with yeast method combined with uricase inhibition method to build hyperuricemia model,and the corresponding drugs（37.5 g/kg,18.75 g/kg,9.375 g/kg, and 0.02 g/kg per day） were administrated on the 7th day.On the 22nd day,the blood uric acid concentration was detected,and microRNA with obvious changes in kidney was screened with qRT-PCR.Results：The uric acid in the model group was higher than that in the control group,and the levels of the uric acid were reduced after being treated with XZCBD; the differences among groups were significant（P〈0.05）.Compared with the control group,32 kinds of microRNA expression changes were detected on the 15th day after being treated with high-dose XZCBD by microRNA expression profile screening.Among them,miR-34a could inhibit the expression of human urate anion exthanger 1,and miR-146a might have inhibited the inflammatory reaction.Conclusion： XZCBD could significantly reduce the serum uric acid level; its effect on hyperuricemia might be through affecting microRNA expressions.