Incoherent holographic imaging of subsurface structures with volume holographic gratings

Under broadband illumination, a multiplane microscopy incorporating volume holographic gratings (VHGs) to observe three-dimensional structures of biological samples with different depth simultaneously is presented. VHGs formed in thick recording materials, including PQ-PMMA, provide strong angular a...

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Bibliographic Details
Main Authors Chia, Yu-Hsin, Wang, Hung-Chun, Luo, Yuan
Format Conference Proceeding
LanguageEnglish
Published SPIE 24.04.2018
Online AccessGet full text
ISBN9781510619791
1510619798
ISSN0277-786X
DOI10.1117/12.2319365

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Summary:Under broadband illumination, a multiplane microscopy incorporating volume holographic gratings (VHGs) to observe three-dimensional structures of biological samples with different depth simultaneously is presented. VHGs formed in thick recording materials, including PQ-PMMA, provide strong angular and wavelength transmittance filtering properties, which enable acquiring spatial–spectral images of fine structures within biological samples. Here, we experimentally demonstrate this microscopic imaging capability to obtain multiple depth-resolved mixed pollen grains images of fine structures from eight depths in one shot.
Bibliography:Conference Date: 2018-04-25|2018-04-27
Conference Location: Yokohama, Japan
ISBN:9781510619791
1510619798
ISSN:0277-786X
DOI:10.1117/12.2319365