蚌肉多糖的提取分离及对UVB损伤的修复作用

TS254.1; 为提高三角帆蚌采珠副产物的利用率,本研究开发利用蚌肉多糖(Hyriopsis cumingii Polysaccharide,HCP),探究了蚌肉多糖对正常细胞的增殖迁移效果和对UVB损伤细胞的修复作用.采用DEAE纤维素-52阴离子交换、葡聚糖G-100凝胶和纳滤法分离纯化蚌肉多糖,采用高效液相色谱、高效凝胶渗透色谱和红外光谱对各组分进行结构检测,并通过细胞增殖实验、细胞划痕实验、UVB损伤实验、蛋白表达检测探究多糖组分的抗UVB损伤效果及作用机制.结果表明,分离纯化得到了三个均一的多糖组分,其中组分2(HCP-2)是蚌肉多糖中对细胞增殖发挥主要作用的组分,且能够促进HaC...

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Published in食品工业科技 Vol. 45; no. 19; pp. 346 - 356
Main Authors 郑雅文, 杨安全, 王菁, 朱松明, 刘鹰, 叶章颖
Format Journal Article
LanguageChinese
Published 浙江大学生物系统工程与食品科学学院,农业农村部设施农业装备与信息化重点实验室,浙江杭州 310058%浙江欧诗漫集团有限公司,浙江湖州 313200 01.10.2024
Subjects
UVB
细胞凋亡
HaCaT细胞
Hyriopsis cumingii
polysaccharide
HaCaT cells
三角帆蚌
多糖
cell apoptosis
Online AccessGet full text
ISSN1002-0306
DOI10.13386/j.issn1002-0306.2023110213

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Abstract TS254.1; 为提高三角帆蚌采珠副产物的利用率,本研究开发利用蚌肉多糖(Hyriopsis cumingii Polysaccharide,HCP),探究了蚌肉多糖对正常细胞的增殖迁移效果和对UVB损伤细胞的修复作用.采用DEAE纤维素-52阴离子交换、葡聚糖G-100凝胶和纳滤法分离纯化蚌肉多糖,采用高效液相色谱、高效凝胶渗透色谱和红外光谱对各组分进行结构检测,并通过细胞增殖实验、细胞划痕实验、UVB损伤实验、蛋白表达检测探究多糖组分的抗UVB损伤效果及作用机制.结果表明,分离纯化得到了三个均一的多糖组分,其中组分2(HCP-2)是蚌肉多糖中对细胞增殖发挥主要作用的组分,且能够促进HaCaT细胞迁移;随着HCP-2浓度的增加,促进UVB损伤细胞活力恢复的效果也越明显;在样品浓度相同的情况下,修复效果优于保护效果;HCP-2上调了抗凋亡蛋白bcl-2表达,抑制了凋亡相关蛋白casp9、p-Akt、p-p38表达,说明HCP-2能够通过调控UVB损伤后的细胞凋亡来修复HaCaT细胞.综上所述,蚌肉多糖能够抑制细胞凋亡,为后续在食品、保健品等领域的开发应用提供理论基础.
AbstractList TS254.1; 为提高三角帆蚌采珠副产物的利用率,本研究开发利用蚌肉多糖(Hyriopsis cumingii Polysaccharide,HCP),探究了蚌肉多糖对正常细胞的增殖迁移效果和对UVB损伤细胞的修复作用.采用DEAE纤维素-52阴离子交换、葡聚糖G-100凝胶和纳滤法分离纯化蚌肉多糖,采用高效液相色谱、高效凝胶渗透色谱和红外光谱对各组分进行结构检测,并通过细胞增殖实验、细胞划痕实验、UVB损伤实验、蛋白表达检测探究多糖组分的抗UVB损伤效果及作用机制.结果表明,分离纯化得到了三个均一的多糖组分,其中组分2(HCP-2)是蚌肉多糖中对细胞增殖发挥主要作用的组分,且能够促进HaCaT细胞迁移;随着HCP-2浓度的增加,促进UVB损伤细胞活力恢复的效果也越明显;在样品浓度相同的情况下,修复效果优于保护效果;HCP-2上调了抗凋亡蛋白bcl-2表达,抑制了凋亡相关蛋白casp9、p-Akt、p-p38表达,说明HCP-2能够通过调控UVB损伤后的细胞凋亡来修复HaCaT细胞.综上所述,蚌肉多糖能够抑制细胞凋亡,为后续在食品、保健品等领域的开发应用提供理论基础.
Abstract_FL To improve the utilization of by-products of the Hyriopsis cumingii,this study investigated the effect of Hyriopsis cumingii polysaccharide(HCP)on the proliferation and migration of normal cells,as well as its reparative effects on ultraviolet radiation-B(UVB)-damaged cells.First,HCP was isolated and purified using DEAE Cellulose-52 column chromatography,Sephadex G-100 column chromatography and nanofiltration.Then,structural analysis was conducted using high-performance liquid chromatography,high-performance gel permeation chromatography,and infrared spectroscopy.Following this,cell proliferation experiments,cell scratch tests,UVB damage experiments,and protein expression analyses were employed to explore the anti-UVB damage effects and corresponding mechanisms of polysaccharide components.The results indicated the three substances obtained by isolation and purification were all homogeneous polysaccharide components.Among them,HCP-2 played a major role in promoting cell proliferation in HCP and facilitated the migration of HaCaT cells.The recovery of UVB-damaged cell vitality was raised by the increasing concentration of HCP-2.Furthermore,the reparative effect was more substantial than the protective effect at the same sample concentration.It was also observed that HCP-2 up-regulated the expression of the anti-apoptotic protein bcl-2 while inhibited the expression of apoptosis-related proteins casp9,p-Akt,and p-p38,indicating that HCP-2 could repair HaCaT cells by regulating apoptosis of cells damaged by UVB.In conclusion,HCP had the ability to inhibit cell apoptosis which provide a theoretical basis for its future application in the development of food and health products.
Author 杨安全
王菁
郑雅文
朱松明
刘鹰
叶章颖
AuthorAffiliation 浙江大学生物系统工程与食品科学学院,农业农村部设施农业装备与信息化重点实验室,浙江杭州 310058%浙江欧诗漫集团有限公司,浙江湖州 313200
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Author_FL LIU Ying
YE Zhangying
ZHU Songming
ZHENG Yawen
YANG Anquan
WANG Jing
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DocumentTitle_FL Extraction and Separation of Hyriopsis cumingii Polysaccharide and the Repairing Effect on UVB Damage
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Issue 19
Keywords UVB
细胞凋亡
HaCaT细胞
Hyriopsis cumingii
polysaccharide
HaCaT cells
三角帆蚌
多糖
cell apoptosis
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