磁性纳米分离技术在食源性致病菌快速检测中的应用

TS207.4; 食源性致病菌是导致食源性疾病的重要因素,对食品安全和人类健康造成了严重危害,是全球卫生保健系统面临的一个巨大挑战.受污染的食品基质复杂且早期致病菌浓度低,干扰了现有检测手段的灵敏度.通常使用传统的增菌培养能提高致病菌的浓度以满足检测需求,但其过程耗时、费力,不能满足市场监管部门快速检测的需要.为了准确检测前期污染食品中的致病菌,保障食品安全,迫切需要食源性致病菌分离富集的有效手段.近年来磁性纳米粒子被广泛研究,通过在其表面修饰能特异性结合致病菌的识别元件,能够对复杂食品基质中的致病菌进行有效的分离富集,结合现有的高灵敏度检测手段,可快速对食源性致病菌进行早期检测.本文主要综述...

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Published in食品工业科技 Vol. 45; no. 11; pp. 361 - 371
Main Authors 杜世龙, 卢菲, 周帆, 王军亮, 肖有玉
Format Journal Article
LanguageChinese
Published 南京市产品质量监督检验院(南京市质量发展与先进技术应用研究院),江苏南京 210019 01.06.2024
Subjects
识别元件
coupling methods
foodborne pathogens
偶联方式
磁性纳米粒子
磁性纳米分离技术
magnetic nanoseparation technology
食源性致病菌
magnetic nanoparticles
recognition elements
Online AccessGet full text
ISSN1002-0306
DOI10.13386/j.issn1002-0306.2023050235

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Abstract TS207.4; 食源性致病菌是导致食源性疾病的重要因素,对食品安全和人类健康造成了严重危害,是全球卫生保健系统面临的一个巨大挑战.受污染的食品基质复杂且早期致病菌浓度低,干扰了现有检测手段的灵敏度.通常使用传统的增菌培养能提高致病菌的浓度以满足检测需求,但其过程耗时、费力,不能满足市场监管部门快速检测的需要.为了准确检测前期污染食品中的致病菌,保障食品安全,迫切需要食源性致病菌分离富集的有效手段.近年来磁性纳米粒子被广泛研究,通过在其表面修饰能特异性结合致病菌的识别元件,能够对复杂食品基质中的致病菌进行有效的分离富集,结合现有的高灵敏度检测手段,可快速对食源性致病菌进行早期检测.本文主要综述了磁性纳米分离技术、磁性纳米粒子与识别元件的偶联方式、识别元件的种类及结合检测手段的应用情况,以期为食源性致病菌快速检测方法的开发提供参考.
AbstractList TS207.4; 食源性致病菌是导致食源性疾病的重要因素,对食品安全和人类健康造成了严重危害,是全球卫生保健系统面临的一个巨大挑战.受污染的食品基质复杂且早期致病菌浓度低,干扰了现有检测手段的灵敏度.通常使用传统的增菌培养能提高致病菌的浓度以满足检测需求,但其过程耗时、费力,不能满足市场监管部门快速检测的需要.为了准确检测前期污染食品中的致病菌,保障食品安全,迫切需要食源性致病菌分离富集的有效手段.近年来磁性纳米粒子被广泛研究,通过在其表面修饰能特异性结合致病菌的识别元件,能够对复杂食品基质中的致病菌进行有效的分离富集,结合现有的高灵敏度检测手段,可快速对食源性致病菌进行早期检测.本文主要综述了磁性纳米分离技术、磁性纳米粒子与识别元件的偶联方式、识别元件的种类及结合检测手段的应用情况,以期为食源性致病菌快速检测方法的开发提供参考.
Abstract_FL Foodbome pathogens are important factors that contribute to foodborne illnesses,posing significant threats to food safety and human health,and presenting a major challenge for global healthcare systems.Contaminated food matrices are complex and often have low concentrations of early-stage pathogens,which hinder the sensitivity of existing detection methods.Traditional microbial culture methods are typically used to increase the concentration of pathogens for detection purposes,but these methods are time-consuming and labor-intensive,making them inadequate for the rapid testing needs of regulatory authorities.Therefore,there is an urgent need for effective methods of isolating and enriching foodbome pathogens to accurately detect early-stage contamination in food and ensure food safety.In recent years,magnetic nanoparticles have been extensively studied.By modifying their surfaces with recognition elements that can specifically bind to pathogens,they can effectively isolate and enrich foodborne pathogens in complex food matrices.When combined with existing highly sensitive detection methods,these magnetic nanoparticles enable rapid early-stage detection of foodborne pathogens.This article provides an overview of Magnetic nanoseparation technology,the coupling methods of magnetic nanoparticles with recognition elements,the types of recognition elements,and the application of combined detection methods.The aim is to provide reference for the development of rapid detection methods for foodborne pathogens.
Author 杜世龙
肖有玉
卢菲
周帆
王军亮
AuthorAffiliation 南京市产品质量监督检验院(南京市质量发展与先进技术应用研究院),江苏南京 210019
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Author_FL ZHOU Fan
DU Shilong
WANG Junliang
XIAO Youyu
LU Fei
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  fullname: XIAO Youyu
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DocumentTitle_FL Application of Magnetic Nanoseparation Technology in Rapid Detection of Foodborne Pathogens
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Issue 11
Keywords 识别元件
coupling methods
foodborne pathogens
偶联方式
磁性纳米粒子
磁性纳米分离技术
magnetic nanoseparation technology
食源性致病菌
magnetic nanoparticles
recognition elements
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PublicationTitle 食品工业科技
PublicationTitle_FL Science and Technology of Food Industry
PublicationYear 2024
Publisher 南京市产品质量监督检验院(南京市质量发展与先进技术应用研究院),江苏南京 210019
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Snippet TS207.4; 食源性致病菌是导致食源性疾病的重要因素,对食品安全和人类健康造成了严重危害,是全球卫生保健系统面临的一个巨大挑战.受污染的食品基质复杂且早期致病菌浓度低,...
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StartPage 361
Title 磁性纳米分离技术在食源性致病菌快速检测中的应用
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