大麦籽粒β-葡聚糖含量的全基因组关联分析及候选基因预测
利用高通量SNP芯片对 238份不同来源的大麦种质资源进行基因型分析,并测定 2年的籽粒β-葡聚糖含量,通过基于PCA模型的一般线性模型(general linear model,GLM)进行全基因组关联分析(genome-wide association study,GWAS).结果表明,238 份大麦材料的β-葡聚糖含量分布在 1.23%～6.55%和 1.79%～6.64%之间且均呈正态分布.GWAS分析共检测到 19 个显著的SNP标记,分布在 1H、2H、3H、4H和 5H染色体上,可解释表型变异的 7.39%～10.29%.在显著关联的SNP位点上下游各 300 kb范围内进行候选...
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| Published in | 作物学报 Vol. 50; no. 10; pp. 2483 - 2492 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
甘肃农业大学农学院,甘肃兰州 730070%省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070
12.10.2024
甘肃农业大学生命科学技术学院,甘肃兰州 730070%甘肃农业大学农学院,甘肃兰州 730070 省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070 |
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| Online Access | Get full text |
| ISSN | 0496-3490 |
| DOI | 10.3724/SP.J.1006.2024.31084 |
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| Abstract | 利用高通量SNP芯片对 238份不同来源的大麦种质资源进行基因型分析,并测定 2年的籽粒β-葡聚糖含量,通过基于PCA模型的一般线性模型(general linear model,GLM)进行全基因组关联分析(genome-wide association study,GWAS).结果表明,238 份大麦材料的β-葡聚糖含量分布在 1.23%～6.55%和 1.79%～6.64%之间且均呈正态分布.GWAS分析共检测到 19 个显著的SNP标记,分布在 1H、2H、3H、4H和 5H染色体上,可解释表型变异的 7.39%～10.29%.在显著关联的SNP位点上下游各 300 kb范围内进行候选基因挖掘,共寻找到 37 个基因,基于前人研究和BLAST基因注释共筛选到 4 个最有可能与β-葡聚糖合成相关的候选基因,在最显著SNP位点B1_1033963 上游 89 kb处找到候选基因HORVU.MOREX.r3.1HG0000140,该基因可能是与β-葡聚糖合成过程紧密相关的基因.本研究可为大麦β-葡聚糖含量遗传改良提供理论指导与优异基因资源. |
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| AbstractList | 利用高通量SNP芯片对 238份不同来源的大麦种质资源进行基因型分析,并测定 2年的籽粒β-葡聚糖含量,通过基于PCA模型的一般线性模型(general linear model,GLM)进行全基因组关联分析(genome-wide association study,GWAS).结果表明,238 份大麦材料的β-葡聚糖含量分布在 1.23%～6.55%和 1.79%～6.64%之间且均呈正态分布.GWAS分析共检测到 19 个显著的SNP标记,分布在 1H、2H、3H、4H和 5H染色体上,可解释表型变异的 7.39%～10.29%.在显著关联的SNP位点上下游各 300 kb范围内进行候选基因挖掘,共寻找到 37 个基因,基于前人研究和BLAST基因注释共筛选到 4 个最有可能与β-葡聚糖合成相关的候选基因,在最显著SNP位点B1_1033963 上游 89 kb处找到候选基因HORVU.MOREX.r3.1HG0000140,该基因可能是与β-葡聚糖合成过程紧密相关的基因.本研究可为大麦β-葡聚糖含量遗传改良提供理论指导与优异基因资源. |
| Abstract_FL | A total of 238 barley germplasm resources from various sources were genotyped using a high-throughput SNP chip.The β-glucan content of barley grain was determined for two consecutive years.Genome-wide association analysis was conducted using a general linear model based on the PCA model.The results showed that the β-glucan content of the 238 barley materials ranged from 1.23%to 6.55%and 1.79%to 6.64%for the respective years,exhibiting a normal distri-bution.In the GWAS analysis,a total of 19 significant SNP markers were detected,located on chromosomes 1H,2H,3H,4H,and 5H.These markers accounted for 7.39%to 10.29%of the observed phenotypic variation.Candidate genes were identified within a 300 kb range upstream and downstream of the SNP sites showing significant associations,and a total of 37 genes were identified.Through a combination of previous studies and BLAST gene annotation,four candidate genes most likely involved in β-glucan synthesis were identified.Notably,within 89 kb upstream of the most significant SNP locus B1_1033963,the candidate gene HORVU.MOREX.R3.1 HG0000140 was detected,which may play a crucial role in the process of beta-glucan synthesis.This study provides theoretical guidance and valuable gene resources for the genetic improvement of β-glucan content in barley. |
| Author | 司二静 尚勋武 马小乐 王化俊 鲁宗辉 姚立蓉 孟亚雄 李葆春 叶霈颖 汪军成 |
| AuthorAffiliation | 省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070;甘肃农业大学农学院,甘肃兰州 730070%省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070;甘肃农业大学生命科学技术学院,甘肃兰州 730070%甘肃农业大学农学院,甘肃兰州 730070 |
| AuthorAffiliation_xml | – name: 省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070;甘肃农业大学农学院,甘肃兰州 730070%省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070;甘肃农业大学生命科学技术学院,甘肃兰州 730070%甘肃农业大学农学院,甘肃兰州 730070 |
| Author_FL | SI Er-Jing MA Xiao-Le SHANG Xun-Wu YE Pei-Yin WANG Jun-Cheng WANG Hua-Jun LI Bao-Chun MENG Ya-Xiong LU Zong-Hui YAO Li-Rong |
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| Keywords | β-glucan 大麦 全基因组关联分析 β-葡聚糖 barley SNP标记 SNP marker genome-wide association analysis |
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| Title | 大麦籽粒β-葡聚糖含量的全基因组关联分析及候选基因预测 |
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