铁皮石斛中性多糖的分离纯化及抗炎作用
TS201.4; 目的:从铁皮石斛中分离纯化得到铁皮石斛多糖,对其进行结构表征,并探究其抗炎作用.方法:以铁皮石斛为原料,通过水提醇沉法得到铁皮石斛粗多糖,再经过DEAE-52纤维素离子交换树脂和葡聚糖凝胶Sephadex G-100从粗多糖中分离纯化得到多糖组分DOP-1.利用紫外扫描法、气相色谱法、高效凝胶色谱、红外光谱和核磁共振扫描等技术对多糖DOP-1进行结构分析,并测定DOP-1对小鼠腹腔巨噬细胞RAW 264.7细胞增殖抑制效果,及其对 LPS诱导的 RAW264.7细胞 NO、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白介素-1β(inter...
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| Published in | 食品工业科技 Vol. 45; no. 8; pp. 39 - 46 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
无限极(中国)有限公司,广东广州 510623%广东工业大学生物医药学院,广东广州 510006
01.04.2024
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1002-0306 |
| DOI | 10.13386/j.issn1002-0306.2023080137 |
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| Abstract | TS201.4; 目的:从铁皮石斛中分离纯化得到铁皮石斛多糖,对其进行结构表征,并探究其抗炎作用.方法:以铁皮石斛为原料,通过水提醇沉法得到铁皮石斛粗多糖,再经过DEAE-52纤维素离子交换树脂和葡聚糖凝胶Sephadex G-100从粗多糖中分离纯化得到多糖组分DOP-1.利用紫外扫描法、气相色谱法、高效凝胶色谱、红外光谱和核磁共振扫描等技术对多糖DOP-1进行结构分析,并测定DOP-1对小鼠腹腔巨噬细胞RAW 264.7细胞增殖抑制效果,及其对 LPS诱导的 RAW264.7细胞 NO、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白介素-1β(interleukin-1β,IL-1β)表达的影响.结果:分离纯化得到的多糖DOP-1为鼠李糖、甘露糖、葡萄糖和半乳糖组成的均一性多糖,单糖摩尔比为 0.6∶9.0∶2.7∶2.9,分子量为 59.2 kDa.DOP-1对RAW 264.7细胞增殖无显著性抑制,与模型组相比,DOP-1能显著抑制NO、TNF-α和IL-1β的表达量.结论:本实验得到的铁皮石斛多糖结构明确,且具有较好的抗炎活性,提取工艺条件具有可行性.本研究可为铁皮石斛多糖在抗炎功效产品的开发利用提供理论依据. |
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| AbstractList | TS201.4; 目的:从铁皮石斛中分离纯化得到铁皮石斛多糖,对其进行结构表征,并探究其抗炎作用.方法:以铁皮石斛为原料,通过水提醇沉法得到铁皮石斛粗多糖,再经过DEAE-52纤维素离子交换树脂和葡聚糖凝胶Sephadex G-100从粗多糖中分离纯化得到多糖组分DOP-1.利用紫外扫描法、气相色谱法、高效凝胶色谱、红外光谱和核磁共振扫描等技术对多糖DOP-1进行结构分析,并测定DOP-1对小鼠腹腔巨噬细胞RAW 264.7细胞增殖抑制效果,及其对 LPS诱导的 RAW264.7细胞 NO、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白介素-1β(interleukin-1β,IL-1β)表达的影响.结果:分离纯化得到的多糖DOP-1为鼠李糖、甘露糖、葡萄糖和半乳糖组成的均一性多糖,单糖摩尔比为 0.6∶9.0∶2.7∶2.9,分子量为 59.2 kDa.DOP-1对RAW 264.7细胞增殖无显著性抑制,与模型组相比,DOP-1能显著抑制NO、TNF-α和IL-1β的表达量.结论:本实验得到的铁皮石斛多糖结构明确,且具有较好的抗炎活性,提取工艺条件具有可行性.本研究可为铁皮石斛多糖在抗炎功效产品的开发利用提供理论依据. |
| Abstract_FL | Objective:To isolate and purify polysaccharides from Dendrobium officinale,characterize their structure and explore their anti-inflammatory effects.Methods:Polysaccharides from Dendrobium officinale were obtained by water extraction and alcohol precipitation.DOP-1 was purified by DEAE-52 cellulose ion exchange resin and Sephadex G-100.Ultraviolet scanning,GC,high-performance gel chromatography,infrared spectroscopy and nuclear magnetic resonance scanning were used to analyze the structure of DOP-1 polysaccharide,and the inhibitory effect of DOP-1 on the proliferation of RAW 264.7 cells was determined.And its effect on the expression of NO,TNF-α and IL-1β in RAW264.7 cells induced by LPS.Results:DOP-1 was a homogeneous polysaccharide composed of rhamnose,mannose,glucose and galactose with a monosaccharide molar ratio of 0.6∶9.0∶2.7∶2.9 and a molecular weight of 59.2 kDa.DOP-1 did not significantly inhibit the proliferation of RAW 264.7 cells.Compared with the model group,DOP-1 significantly inhibited the expression of NO,TNF-α and IL-1β.Conclusions:The polysaccharides of Dendrobium officinaleobtained in this study had clear structure and good anti-inflammatory activity,and the extraction conditions were feasible.This study would provide theoretical basis for the development and utilization of polysaccharides of Dendrobium officinale with anti-inflammatory effect. |
| Author | 谢玲娜 梁明 梁逸恒 杜志云 陈梦香 林晓亮 |
| AuthorAffiliation | 无限极(中国)有限公司,广东广州 510623%广东工业大学生物医药学院,广东广州 510006 |
| AuthorAffiliation_xml | – name: 无限极(中国)有限公司,广东广州 510623%广东工业大学生物医药学院,广东广州 510006 |
| Author_FL | LIN Xiaoliang CHEN Mengxiang XIE Lingna LIANG Ming DU Zhiyun LIANG Yiheng |
| Author_FL_xml | – sequence: 1 fullname: LIN Xiaoliang – sequence: 2 fullname: XIE Lingna – sequence: 3 fullname: CHEN Mengxiang – sequence: 4 fullname: LIANG Ming – sequence: 5 fullname: LIANG Yiheng – sequence: 6 fullname: DU Zhiyun |
| Author_xml | – sequence: 1 fullname: 林晓亮 – sequence: 2 fullname: 谢玲娜 – sequence: 3 fullname: 陈梦香 – sequence: 4 fullname: 梁明 – sequence: 5 fullname: 梁逸恒 – sequence: 6 fullname: 杜志云 |
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| DocumentTitle_FL | Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral Polysaccharide |
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| Keywords | neutral polysaccharide isolation and purification 抗炎作用 structural analysis Dendrobium officinale 中性多糖 anti-inflammatory effect 分离纯化 铁皮石斛 结构分析 |
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| Publisher | 无限极(中国)有限公司,广东广州 510623%广东工业大学生物医药学院,广东广州 510006 |
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| Title | 铁皮石斛中性多糖的分离纯化及抗炎作用 |
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