猴头菇三萜提取工艺优化及其抗氧化活性分析
S646.9; 为确定猴头菇三萜最佳提取工艺参数,通过Box-Behnken响应面法设计,以乙醇浓度、提取温度、料液比为影响因素,猴头菇三萜得率为响应值,应用Design expert 10.0.3软件进行二次多项式拟合,绘制等高线图和响应面图,确定最佳提取条件,并对其进行抗氧化活性测定.结果表明,最佳提取工艺条件为:乙醇浓度60％,温度55℃,料液比1:15(g:mL),验证试验显示猴头菇三萜得率为0.28％,与预测值接近,说明响应曲面法建立的模型准确可靠,能合理优化猴头菇三萜的提取工艺.DPPH·清除能力、还原力、ABTS+·清除能力3种抗氧化体系综合评价表明,提取物浓度与自由基清除力呈良...
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| Published in | 食品工业科技 Vol. 42; no. 22; pp. 153 - 159 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
浙江省农业科学院 食品科学研究所,农业农村部果品采后处理重点实验室,浙江省果蔬保鲜与加工技术研究重点实验室,浙江杭州 310021%浙江工业大学食品科学与工程学院,浙江杭州 310014%常山县豪锋农业发展有限公司,浙江常山 324207
01.11.2021
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1002-0306 |
| DOI | 10.13386/j.issn1002-0306.2020040354 |
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| Abstract | S646.9; 为确定猴头菇三萜最佳提取工艺参数,通过Box-Behnken响应面法设计,以乙醇浓度、提取温度、料液比为影响因素,猴头菇三萜得率为响应值,应用Design expert 10.0.3软件进行二次多项式拟合,绘制等高线图和响应面图,确定最佳提取条件,并对其进行抗氧化活性测定.结果表明,最佳提取工艺条件为:乙醇浓度60％,温度55℃,料液比1:15(g:mL),验证试验显示猴头菇三萜得率为0.28％,与预测值接近,说明响应曲面法建立的模型准确可靠,能合理优化猴头菇三萜的提取工艺.DPPH·清除能力、还原力、ABTS+·清除能力3种抗氧化体系综合评价表明,提取物浓度与自由基清除力呈良好的剂量关系.猴头菇三萜提取物对DPPH和ABTS+自由基半抑制浓度(IC50)分别为(6.375±0.020)mg·mL-1和(0.355±0.040)mg·mL-1,其具有一定的抗氧化性. |
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| AbstractList | S646.9; 为确定猴头菇三萜最佳提取工艺参数,通过Box-Behnken响应面法设计,以乙醇浓度、提取温度、料液比为影响因素,猴头菇三萜得率为响应值,应用Design expert 10.0.3软件进行二次多项式拟合,绘制等高线图和响应面图,确定最佳提取条件,并对其进行抗氧化活性测定.结果表明,最佳提取工艺条件为:乙醇浓度60％,温度55℃,料液比1:15(g:mL),验证试验显示猴头菇三萜得率为0.28％,与预测值接近,说明响应曲面法建立的模型准确可靠,能合理优化猴头菇三萜的提取工艺.DPPH·清除能力、还原力、ABTS+·清除能力3种抗氧化体系综合评价表明,提取物浓度与自由基清除力呈良好的剂量关系.猴头菇三萜提取物对DPPH和ABTS+自由基半抑制浓度(IC50)分别为(6.375±0.020)mg·mL-1和(0.355±0.040)mg·mL-1,其具有一定的抗氧化性. |
| Author | 夏其乐 毛荣良 李英迪 杨开 曹艳 |
| AuthorAffiliation | 浙江省农业科学院 食品科学研究所,农业农村部果品采后处理重点实验室,浙江省果蔬保鲜与加工技术研究重点实验室,浙江杭州 310021%浙江工业大学食品科学与工程学院,浙江杭州 310014%常山县豪锋农业发展有限公司,浙江常山 324207 |
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| Author_FL | LI Yingdi CAO Yan XIA Qile YANG Kai MAO Rongliang |
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| Keywords | 猴头菇;三萜;提取;响应面法;抗氧化性 |
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| Snippet | S646.9; 为确定猴头菇三萜最佳提取工艺参数,通过Box-Behnken响应面法设计,以乙醇浓度、提取温度、料液比为影响因素,猴头菇三萜得率为响应值,应用Design expert 10.0.3软件进... |
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| Title | 猴头菇三萜提取工艺优化及其抗氧化活性分析 |
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