光调控红色红曲菌M7及MrVeA基因相关突变株的功能分析

TS201.3; 为了探讨光调控对红色红曲菌(Monascus ruber)M7、MrVeA基因相关突变株ΔMrVeA、OEMrVeA功能的影响,采用MrVeA基因敲除、过表达技术分别构建基因缺失菌株(ΔMrVeA)和过表达菌株(OEMrVeA).以避光(黑暗)为对照,分析红光和白光对红色红曲菌M7、MrVeA基因突变株ΔMrVeA、OEMrVeA生长发育、红曲色素(Mps)和桔霉素(CIT)产生的影响.结果表明,黑暗条件下,突变株OEMrVeA闭囊壳和分生孢子的产量增加,菌丝发育、菌落颜色、生物量及色素含量与红色红曲菌(M.ruber)M7相比均无显著性差异,但产桔霉素能力比菌株M7降低7....

Full description

Saved in:
Bibliographic Details
Published in中国酿造 Vol. 43; no. 5; pp. 53 - 61
Main Authors 赵玉霞, 何霞, 王丽玲, 黄汇惠, 匡林莎, 何毅
Format Journal Article
LanguageChinese
Published 塔里木大学 食品科学与工程学院,新疆 阿拉尔 843300%武汉轻工大学 硒科学与工程现代产业学院国家富硒农产品加工技术研发专业中心 湖北省绿色富硒农产品精深加工工程技术研究中心,湖北 武汉 430023 25.05.2024
Subjects
桔霉素
citrinin
Monascus pigment
红曲菌
天鹅绒蛋白
velvet protein
Monascus
红曲色素
光调控
light control
Online AccessGet full text
ISSN0254-5071
DOI10.11882/j.issn.0254-5071.2024.05.009

Cover

Abstract TS201.3; 为了探讨光调控对红色红曲菌(Monascus ruber)M7、MrVeA基因相关突变株ΔMrVeA、OEMrVeA功能的影响,采用MrVeA基因敲除、过表达技术分别构建基因缺失菌株(ΔMrVeA)和过表达菌株(OEMrVeA).以避光(黑暗)为对照,分析红光和白光对红色红曲菌M7、MrVeA基因突变株ΔMrVeA、OEMrVeA生长发育、红曲色素(Mps)和桔霉素(CIT)产生的影响.结果表明,黑暗条件下,突变株OEMrVeA闭囊壳和分生孢子的产量增加,菌丝发育、菌落颜色、生物量及色素含量与红色红曲菌(M.ruber)M7相比均无显著性差异,但产桔霉素能力比菌株M7降低7.03%,说明MrVeA基因的过表达会促进菌株M7的有性/无性繁殖,抑制桔霉素的生成.红光有利于突变株OEMrVeA的生长发育、有性/无性繁殖、红曲色素的生成,不利于突变株OEMrVeA生成桔霉素;白光不利于突变株OEMrVeA的生长发育、有性/无性繁殖、红曲色素的生成.研究结果可为低产甚至不产桔霉素的红曲菌工业化应用提供参考.
AbstractList TS201.3; 为了探讨光调控对红色红曲菌(Monascus ruber)M7、MrVeA基因相关突变株ΔMrVeA、OEMrVeA功能的影响,采用MrVeA基因敲除、过表达技术分别构建基因缺失菌株(ΔMrVeA)和过表达菌株(OEMrVeA).以避光(黑暗)为对照,分析红光和白光对红色红曲菌M7、MrVeA基因突变株ΔMrVeA、OEMrVeA生长发育、红曲色素(Mps)和桔霉素(CIT)产生的影响.结果表明,黑暗条件下,突变株OEMrVeA闭囊壳和分生孢子的产量增加,菌丝发育、菌落颜色、生物量及色素含量与红色红曲菌(M.ruber)M7相比均无显著性差异,但产桔霉素能力比菌株M7降低7.03%,说明MrVeA基因的过表达会促进菌株M7的有性/无性繁殖,抑制桔霉素的生成.红光有利于突变株OEMrVeA的生长发育、有性/无性繁殖、红曲色素的生成,不利于突变株OEMrVeA生成桔霉素;白光不利于突变株OEMrVeA的生长发育、有性/无性繁殖、红曲色素的生成.研究结果可为低产甚至不产桔霉素的红曲菌工业化应用提供参考.
Abstract_FL In order to investigate the effect of light control on the function of Monascus ruber M7 and MrVeA gene related mutant ΔMrVeA,OEMrVeA,the gene deletion strain(ΔMrVeA)and overexpression strain(OEMrVeA)were constructed by MrVeA gene knockout and overexpression techniques,respectively.Using lucifuge(dark)as control,the effects of red light and white light on the growth and development,and the production of Monascus pigment(Mps)and citrinin(CIT)of M.ruber M7,MrVeA gene mutant strains ΔMrVeA and OEMrVeA were analyzed.The results showed that the production of cleistothecium and conidium of mutant OEMrVeA increased under dark conditions,and there was no significant difference in mycelium development,colony color,biomass and pigment content compared with M.ruber M7,but the citrinin production capacity decreased by 7.03%com-pared with M.ruber M7,indicating that the overexpression of MrVeA gene could promote the sexual/asexual reproduction of strain M.ruber M7 and inhibit the citrinin formation.Red light was conducive to the growth and development of the mutant OEMrVeA,sexual/asexual reproduction and Mps generation,but was not conducive to citrinin generation of mutant strain OEMrVeA.White light was not conducive to the growth and development of mutant OEMrVeA,sexual/asexual reproduction,and Mps generation.The results could provide a reference for the industrial application of Monascus with low or even no citrinin production.
Author 何毅
何霞
赵玉霞
王丽玲
黄汇惠
匡林莎
AuthorAffiliation 塔里木大学 食品科学与工程学院,新疆 阿拉尔 843300%武汉轻工大学 硒科学与工程现代产业学院国家富硒农产品加工技术研发专业中心 湖北省绿色富硒农产品精深加工工程技术研究中心,湖北 武汉 430023
AuthorAffiliation_xml – name: 塔里木大学 食品科学与工程学院,新疆 阿拉尔 843300%武汉轻工大学 硒科学与工程现代产业学院国家富硒农产品加工技术研发专业中心 湖北省绿色富硒农产品精深加工工程技术研究中心,湖北 武汉 430023
Author_FL ZHAO Yuxia
KUANG Linsha
HE Xia
HUANG Huihui
HE Yi
WANG Liling
Author_FL_xml – sequence: 1
  fullname: ZHAO Yuxia
– sequence: 2
  fullname: HE Xia
– sequence: 3
  fullname: WANG Liling
– sequence: 4
  fullname: HUANG Huihui
– sequence: 5
  fullname: KUANG Linsha
– sequence: 6
  fullname: HE Yi
Author_xml – sequence: 1
  fullname: 赵玉霞
– sequence: 2
  fullname: 何霞
– sequence: 3
  fullname: 王丽玲
– sequence: 4
  fullname: 黄汇惠
– sequence: 5
  fullname: 匡林莎
– sequence: 6
  fullname: 何毅
BookMark eNo9jz1Lw0Acxm-oYFv9GB0T_3eXS3JjKVaFFhd1LZfkUgxyhQYRukmt2CLGUSzY4iJ0krpIwY9zST6GJ4rL84NneF5qqKIGSiLUwGBj7PtkL7HP01TZQJhjMfCwTYA4NjAbgFdQ9d_fRrU0TQAodz2ooraeTMv3cf7wVmxey-naaD5fl9l919PZrDs8k0292Oj5sph_6slHsbrW2VO-XBXPN3q2KMdf-u42f3ncQVuxuEjl7h_r6LS9f9I6tDrHB0etZsdKsdlhRSG4VHgO507AGZMEh34oGfYxhIGh9Dh1eRwFgcP8OOSBMNdCFkXUpUS4lNZR4zf3SqhYqH4vGVwOlWnsjfpq9HMZmCmi33oHZdY
ClassificationCodes TS201.3
ContentType Journal Article
Copyright Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
Copyright_xml – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
DBID 2B.
4A8
92I
93N
PSX
TCJ
DOI 10.11882/j.issn.0254-5071.2024.05.009
DatabaseName Wanfang Data Journals - Hong Kong
WANFANG Data Centre
Wanfang Data Journals
万方数据期刊 - 香港版
China Online Journals (COJ)
China Online Journals (COJ)
DatabaseTitleList
DeliveryMethod fulltext_linktorsrc
Discipline Engineering
DocumentTitle_FL Functional analysis of Monascus ruber M7 and MrVeAgene related mutants by light control
EndPage 61
ExternalDocumentID zgnz202405009
GrantInformation_xml – fundername: (国家自然科学基金); (兵团财政科技计划); (图木舒克市科技计划项目)
  funderid: (国家自然科学基金); (兵团财政科技计划); (图木舒克市科技计划项目)
GroupedDBID -02
2B.
4A8
5XA
5XC
92H
92I
93N
ABJNI
ACGFS
ALMA_UNASSIGNED_HOLDINGS
CCEZO
CDRFL
CW9
GROUPED_DOAJ
PSX
TCJ
TGT
U1G
U5L
ID FETCH-LOGICAL-s1009-dc063a74994b955e21c8ce51810cbe51e79369fdbb458fc9ba882c5dd3632a633
ISSN 0254-5071
IngestDate Thu May 29 04:05:40 EDT 2025
IsPeerReviewed false
IsScholarly true
Issue 5
Keywords 桔霉素
citrinin
Monascus pigment
红曲菌
天鹅绒蛋白
velvet protein
Monascus
红曲色素
光调控
light control
Language Chinese
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-s1009-dc063a74994b955e21c8ce51810cbe51e79369fdbb458fc9ba882c5dd3632a633
PageCount 9
ParticipantIDs wanfang_journals_zgnz202405009
PublicationCentury 2000
PublicationDate 2024-05-25
PublicationDateYYYYMMDD 2024-05-25
PublicationDate_xml – month: 05
  year: 2024
  text: 2024-05-25
  day: 25
PublicationDecade 2020
PublicationTitle 中国酿造
PublicationTitle_FL China Brewing
PublicationYear 2024
Publisher 塔里木大学 食品科学与工程学院,新疆 阿拉尔 843300%武汉轻工大学 硒科学与工程现代产业学院国家富硒农产品加工技术研发专业中心 湖北省绿色富硒农产品精深加工工程技术研究中心,湖北 武汉 430023
Publisher_xml – name: 塔里木大学 食品科学与工程学院,新疆 阿拉尔 843300%武汉轻工大学 硒科学与工程现代产业学院国家富硒农产品加工技术研发专业中心 湖北省绿色富硒农产品精深加工工程技术研究中心,湖北 武汉 430023
SSID ssj0039670
ssib002263015
ssib001104799
ssib051369242
Score 2.3848023
Snippet TS201.3; 为了探讨光调控对红色红曲菌(Monascus ruber)M7、MrVeA基因相关突变株ΔMrVeA、OEMrVeA功能的影响,采用MrVeA基因敲除、过表达技术分别构建基因缺失菌株(ΔMrVeA)和过...
SourceID wanfang
SourceType Aggregation Database
StartPage 53
Title 光调控红色红曲菌M7及MrVeA基因相关突变株的功能分析
URI https://d.wanfangdata.com.cn/periodical/zgnz202405009
Volume 43
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAON
  databaseName: DOAJ Directory of Open Access Journals
  issn: 0254-5071
  databaseCode: DOA
  dateStart: 20240101
  customDbUrl:
  isFulltext: true
  dateEnd: 99991231
  titleUrlDefault: https://www.doaj.org/
  omitProxy: true
  ssIdentifier: ssj0039670
  providerName: Directory of Open Access Journals
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Na9RAFA_FguhB_MTP0oNzWrZmMx_JHCfbhCKsp1Z6K_lqPa3Qj8vepFZsEetRLNjiRehJ6kUK_jnZ3ZN_g--9pNnULtUKSzqdefM-fu8l-2ayM2NZj_EwcteLVTNy06wpklg3o1TaTelGMhM8Sr0U3-h2nqm5BfF0US5OTP6q_WppYz2eSXpj15X8j1ehDvyKq2Qv4NmKKVRAGfwLV_AwXP_JxyyQzIOPZoHHfJt5nAWKeQEzLgtc5htmHGwCAt-p1SimfaqBppB57Y5LnKBoOqvPM4P_6RCpseAzY2Nn7OOVIn2ONcZAIlp21R7yBUqoRGJoEtRkkBVK4syfpRooK1IiYMUhnifZMQsEijCzpVyk1yQupAIwr96ukMkSPyANTEYQNNNt4DoiEchCyzEtRR-_lIiCqMZ3RiQAmk9GKOa3mOcStvxEhXKmxBH4kr9YVU2xjaqbFtOCNAambTK1zQxhZwQ5R6KRRjWQyHBCCCzmBKeLFLpVEZU6gnZQ40MrmQzsUP2KBizU6FynkKcwHtAPoHMhBhyEMCrshmBJpNCi4QlOR3VL8l_BrLC4CKtZhGEkeYwJLhnsXFDzAm-baHwEAQuGWFMvbcZYN4oLaILuigohoVypITG-APERwwpbikcM50ofhTUQWaWTQuJjk_-KuG6d6MNritXiFED1eIMw0wg3doNYozsQOBYeBQv9sHYrXkhpF7v4AYlw0TNjzDiL7zmGQUETdn-aUUbPaUMaZwOjITim4bUswpGiiYO-esojeO3RLmv5S7Fxd5kJF6cknM2xYFBOSRbyn6n4z-AtR7sg23qUXFY_-e2tdHtIYUtabz3pQBZm16bAaPiG-8bUp0ccxWvDFdniSjui2h6Qa-UWc-ilCpctdqLgk_PUowWa3eWou1IbS8xft66VkwDTpnii37Amei9uWldrW8PessJ8a3v4bbP__uvg-Mtw-wiu_b2j4e67jpvv7tBTOt8_zvcOBns_8q3vg8NX-e7H_sHh4NPrfGd_uPkzf_um__nDbWshDObbc83yzJvmWgvfU6cJjBkjV2gtYi1l5rQSL8kkjMPsJIa_mYsnsC6ncSykt5zoOAJjE5mmXHEnUpzfsS51X3azu9Y0kEbKiVPpyFRkScuLbDvhOEUgvcSN-D1rqkRhqfxOW1s65aX7fyN4YF0ZPWcfWpfWVzeyRzBGW4-nyLG_ASAkEhw
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%85%89%E8%B0%83%E6%8E%A7%E7%BA%A2%E8%89%B2%E7%BA%A2%E6%9B%B2%E8%8F%8CM7%E5%8F%8AMrVeA%E5%9F%BA%E5%9B%A0%E7%9B%B8%E5%85%B3%E7%AA%81%E5%8F%98%E6%A0%AA%E7%9A%84%E5%8A%9F%E8%83%BD%E5%88%86%E6%9E%90&rft.jtitle=%E4%B8%AD%E5%9B%BD%E9%85%BF%E9%80%A0&rft.au=%E8%B5%B5%E7%8E%89%E9%9C%9E&rft.au=%E4%BD%95%E9%9C%9E&rft.au=%E7%8E%8B%E4%B8%BD%E7%8E%B2&rft.au=%E9%BB%84%E6%B1%87%E6%83%A0&rft.date=2024-05-25&rft.pub=%E5%A1%94%E9%87%8C%E6%9C%A8%E5%A4%A7%E5%AD%A6+%E9%A3%9F%E5%93%81%E7%A7%91%E5%AD%A6%E4%B8%8E%E5%B7%A5%E7%A8%8B%E5%AD%A6%E9%99%A2%2C%E6%96%B0%E7%96%86+%E9%98%BF%E6%8B%89%E5%B0%94+843300%25%E6%AD%A6%E6%B1%89%E8%BD%BB%E5%B7%A5%E5%A4%A7%E5%AD%A6+%E7%A1%92%E7%A7%91%E5%AD%A6%E4%B8%8E%E5%B7%A5%E7%A8%8B%E7%8E%B0%E4%BB%A3%E4%BA%A7%E4%B8%9A%E5%AD%A6%E9%99%A2%E5%9B%BD%E5%AE%B6%E5%AF%8C%E7%A1%92%E5%86%9C%E4%BA%A7%E5%93%81%E5%8A%A0%E5%B7%A5%E6%8A%80%E6%9C%AF%E7%A0%94%E5%8F%91%E4%B8%93%E4%B8%9A%E4%B8%AD%E5%BF%83+%E6%B9%96%E5%8C%97%E7%9C%81%E7%BB%BF%E8%89%B2%E5%AF%8C%E7%A1%92%E5%86%9C%E4%BA%A7%E5%93%81%E7%B2%BE%E6%B7%B1%E5%8A%A0%E5%B7%A5%E5%B7%A5%E7%A8%8B%E6%8A%80%E6%9C%AF%E7%A0%94%E7%A9%B6%E4%B8%AD%E5%BF%83%2C%E6%B9%96%E5%8C%97+%E6%AD%A6%E6%B1%89+430023&rft.issn=0254-5071&rft.volume=43&rft.issue=5&rft.spage=53&rft.epage=61&rft_id=info:doi/10.11882%2Fj.issn.0254-5071.2024.05.009&rft.externalDocID=zgnz202405009
thumbnail_s http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fzgnz%2Fzgnz.jpg