高产乙酸乙酯酵母菌的筛选、鉴定及发酵条件优化
TS262.3; 该研究以产酯酵母为研究对象,通过气相色谱-质谱联用(GC-MS)技术测定菌株的乙酸乙酯产量并筛选高产乙酸乙酯的酵母菌株,通过形态学观察和分子生物学技术对其进行菌种鉴定,并以乙酸乙酯产量为响应值,通过单因素及响应面试验对其发酵条件进行优化.结果表明,筛选得到一株高产乙酸乙酯的酵母菌株P-3,乙酸乙酯产量为134.32 mg/L.菌株P-3被鉴定为库德里阿兹威毕赤酵母(Pichia kudriavzevii),其产乙酸乙酯的最佳发酵条件为:发酵温度30℃,乙醇体积分数18%,发酵时间4 d,在此条件下,乙酸乙酯产量达到281.47 mg/L,比优化前提高109.55%....
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| Published in | 中国酿造 Vol. 43; no. 5; pp. 105 - 110 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
湖北工业大学 生物工程与食品学院 工业发酵省部共建协同创新中心 发酵工程教育部重点实验室工业微生物湖北省重点实验室,湖北 武汉 430068
25.05.2024
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0254-5071 |
| DOI | 10.11882/j.issn.0254-5071.2024.05.016 |
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| Abstract | TS262.3; 该研究以产酯酵母为研究对象,通过气相色谱-质谱联用(GC-MS)技术测定菌株的乙酸乙酯产量并筛选高产乙酸乙酯的酵母菌株,通过形态学观察和分子生物学技术对其进行菌种鉴定,并以乙酸乙酯产量为响应值,通过单因素及响应面试验对其发酵条件进行优化.结果表明,筛选得到一株高产乙酸乙酯的酵母菌株P-3,乙酸乙酯产量为134.32 mg/L.菌株P-3被鉴定为库德里阿兹威毕赤酵母(Pichia kudriavzevii),其产乙酸乙酯的最佳发酵条件为:发酵温度30℃,乙醇体积分数18%,发酵时间4 d,在此条件下,乙酸乙酯产量达到281.47 mg/L,比优化前提高109.55%. |
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| AbstractList | TS262.3; 该研究以产酯酵母为研究对象,通过气相色谱-质谱联用(GC-MS)技术测定菌株的乙酸乙酯产量并筛选高产乙酸乙酯的酵母菌株,通过形态学观察和分子生物学技术对其进行菌种鉴定,并以乙酸乙酯产量为响应值,通过单因素及响应面试验对其发酵条件进行优化.结果表明,筛选得到一株高产乙酸乙酯的酵母菌株P-3,乙酸乙酯产量为134.32 mg/L.菌株P-3被鉴定为库德里阿兹威毕赤酵母(Pichia kudriavzevii),其产乙酸乙酯的最佳发酵条件为:发酵温度30℃,乙醇体积分数18%,发酵时间4 d,在此条件下,乙酸乙酯产量达到281.47 mg/L,比优化前提高109.55%. |
| Abstract_FL | In this study,the ester-producing yeast was used as the research object,the ethyl acetate yield was determined by gas chromatography-mass spectrometry(GC-MS)technology,and the yeast strains with high ethyl acetate yield were screened.The strains were identified by morphological ob-servation and molecular biology techniques,and the fermentation conditions were optimized by single factor and response surface experiments with the ethyl acetate yield as the response value.The results showed that a high-yielding ethyl acetate yeast strain P-3 was obtained,with an ethyl acetate yield of 134.32 mg/L.Strain P-3 was identified as Pichia kudriavzevii,and the optimal fermentation conditions for ethyl acetate production were ob-tained as follows:fermentation temperature 30℃,ethanol volume fraction 18%,and fermentation time 4 d.Under these conditions,the yield of ethyl acetate reached 281.47 mg/L,which was 109.55%higher than that before optimization. |
| Author | 肖雨荷 刘智文 汪江波 朱家豪 蔡晶钰 冉霞 余启 |
| AuthorAffiliation | 湖北工业大学 生物工程与食品学院 工业发酵省部共建协同创新中心 发酵工程教育部重点实验室工业微生物湖北省重点实验室,湖北 武汉 430068 |
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| Author_FL | YU Qi ZHU Jiahao LIU Zhiwen XIAO Yuhe RAN Xia CAI Jingyu WANG Jiangbo |
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| DocumentTitle_FL | Screening,identification and fermentation conditions optimization of high-yielding ethyl acetate yeast |
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| Keywords | 筛选 screening identification 鉴定 response surface analysis ethyl acetate 响应面分析 酵母菌 乙酸乙酯 yeast |
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| Publisher | 湖北工业大学 生物工程与食品学院 工业发酵省部共建协同创新中心 发酵工程教育部重点实验室工业微生物湖北省重点实验室,湖北 武汉 430068 |
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| Snippet | TS262.3; 该研究以产酯酵母为研究对象,通过气相色谱-质谱联用(GC-MS)技术测定菌株的乙酸乙酯产量并筛选高产乙酸乙酯的酵母菌株,通过形态学观察和分子生物学技术对其进行菌... |
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| Title | 高产乙酸乙酯酵母菌的筛选、鉴定及发酵条件优化 |
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