Expression of miR-106b-5p in children with primary immune thrombocytopenia and its correlation with T cells

• Published in: Zhongguo dang dai er ke za zhi Vol. 24; no. 4; p. 411
• Main Authors: Wang, Wen-Fang, Wang, Xu-Song, Tan, San-Yang, Zhong, Lan-Lan, Chen, Jiang
• Format: Journal Article
• Language: Chinese
• Published: China 15.04.2022
• Subjects: Child; Humans; Lymphocyte Count; MicroRNAs - genetics; Purpura, Thrombocytopenic, Idiopathic - genetics; T-Lymphocytes, Regulatory; Th17 Cells; Primary immune thrombocytopenia; Child; T helper 17 cell; miR-106b-5p; Regulatory T cell
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2110139

To study the expression level of plasma miR-106b-5p in primary immune thrombocytopenia (ITP) and its correlation with the levels of T helper 17 cell (Th17) and regulatory T cell (Treg) and the Th17/Treg ratio. A total of 79 children with ITP (ITP group) and 40 healthy children (control group) were selected as subjects. According to the treatment response, the 79 children with ITP were divided into three groups: complete response ( =40), partial response ( =18), and non-response ( =21). Quantitative real-time PCR was used to measure the expression level of miR-106b-5p. Flow cytometry was used to measure the frequencies of Th17 and Treg, and the Th17/Treg ratio was calculated. The correlation of the expression level of plasma miR-106b-5p with the frequencies of Th17 and Treg and the Th17/Treg ratio was analyzed. Compared with the control group, the ITP group had significantly higher levels of miR-106b-5p, Th17, and Th17/Treg ratio ( <0.05) and a significantly lower level of Treg ( <0.05). After treatment, the I