Danshen Injection inhibits peritoneal dialysis fluid-induced endothelial-mesenchymal transition in HMrSV5 cells by regulating the TGF-β/Smad signaling pathway

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 44; no. 12; p. 2276
• Main Authors: Yu, Lihua, Li, Jingya, Wang, Xiaoqi, Li, Li, Chen, Ya, Wang, Feiyu, Zhang, Kun, Wang, Tongsheng
• Format: Journal Article
• Language: Chinese
• Published: China 20.12.2024
• Subjects: Actins - metabolism; Cadherins - metabolism; Cell Line; Dialysis Solutions; Drugs, Chinese Herbal - pharmacology; Endothelial-Mesenchymal Transition; Epithelial-Mesenchymal Transition - drug effects; Humans; Interleukin-6 - metabolism; Peritoneal Dialysis - adverse effects; Salvia miltiorrhiza; Signal Transduction - drug effects; Smad Proteins - metabolism; Transforming Growth Factor beta - metabolism; Tumor Necrosis Factor-alpha - metabolism; Vascular Endothelial Growth Factor A - metabolism; endothelial-mesenchymal transition; peritoneal fibrosis; peritoneal dialysate; TGF‑β/Smad signaling pathway; Danshen Injection
• ISSN: 1673-4254
• DOI: 10.12122/j.issn.1673-4254.2024.12.02

To investigate the inhibitory effect of Danshen Injection on endothelial-mesenchymal transition (EndMT) induced by peritoneal dialysis fluid in HMrSV5 cells and the role of the TGF‑β/Smad signaling pathway in mediating this effect. HMrSV5 cells cultured in 40% peritoneal dialysis solution for 72 h to induce EndMT were treated with 0.05%, 0.1% and 0.5% Danshen Injection. CCK-8 assay was used to assess the changes in viability of the treated cells, and the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), and vascular endothelial growth factor (VEGF) in the cell supernatant were detected using ELISA; Western blotting was performed to detect the protein expressions of E-cadherin, α-smooth muscle actin (α-SMA), p-Smad 2/3, and Smad 7 in the cells. Culture in 40% peritoneal dialysis fluid for 72 induced significant EndMT in HMrSV5 cells, which exhibited obviously lowered cell viability. Danshen Injection within the concentration range of 0.025%-1.5% did not sign