Occurrence of trypsin-like protease in cardamom (Elettaria cardamomum Maton)

Occurrence of trypsin-like protease in fresh cardamom (Elettaria cardamomum Maton) seeds, as evidenced by the benzoyl-arg-p-nitroanilide (BApNA) hydrolyzing ability of the seed enzyme preparation under alkaline condition is reported for the first time. The enzyme has a pH and temperature optima as 8...

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Bibliographic Details
Published inIndian journal of biochemistry & biophysics Vol. 42; no. 4; p. 243
Main Authors Josephrajkumar, A, Chakrabarty, Romit, Thomas, George
Format Journal Article
LanguageEnglish
Published India 01.08.2005
Subjects
Aniline Compounds - metabolism
Aprotinin - pharmacology
Elettaria - drug effects
Elettaria - enzymology
Phenylmethylsulfonyl Fluoride - pharmacology
Seeds - drug effects
Seeds - enzymology
Substrate Specificity
Trypsin - chemistry
Trypsin - metabolism
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ISSN0301-1208

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Summary:Occurrence of trypsin-like protease in fresh cardamom (Elettaria cardamomum Maton) seeds, as evidenced by the benzoyl-arg-p-nitroanilide (BApNA) hydrolyzing ability of the seed enzyme preparation under alkaline condition is reported for the first time. The enzyme has a pH and temperature optima as 8 and 45 degrees C, respectively. It is inhibited by aprotinin and phenylmethyl sulfonyl fluoride (PMSF) in a dose-dependent manner, suggesting the presence of serine residues at the active site. The enzyme had a V(max) of 98.01 nmoles p-nitroaniline released per min per mg protein and K(m) of 0.0684 mM with BApNA as substrate. Addition of aprotinin (75.75 microM) increased K(m) value by three-folds, whereas the V(max) was reduced by 23%.
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ISSN:0301-1208