Role of mu-calpain in human decidua for recurrent miscarriage

• Published in: American journal of reproductive immunology (1989) Vol. 59; no. 4; p. 339
• Main Authors: Kumagai, Kyoko, Ozaki, Yasuhiko, Nakanishi, Tamao, Inomata, Mitsushi, Furuno, Tadahide, Nakanishi, Mamoru, Ogasawara, Mayumi S
• Format: Journal Article
• Language: English
• Published: Denmark 01.04.2008
• Subjects: Abortion, Habitual - metabolism; Adult; Blotting, Western; Calpain - analysis; Calpain - metabolism; Decidua - cytology; Decidua - metabolism; Electrophoresis, Polyacrylamide Gel; Female; Humans; Immunohistochemistry; Proteins - analysis
• ISSN: 1046-7408
• DOI: 10.1111/j.1600-0897.2007.00576.x

Earlier, we showed that calpain activated by increasing intracellular calcium ion concentration in endometrial cells, causes endometrial dysfunction for implantation and early pregnancy. In the present study, we investigated the existence and distribution of calpains, calpastatin, integrin beta3 and alpha-fodrin in decidua from patients with recurrent miscarriage. Deciduae were surgically collected from 29 patients with recurrent miscarriage and 20 healthy women with informed consent. Immunohistochemistry, sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and western blot analysis were performed. Staining of mu-calpain, m-calpain, calpastatin, integrin beta3 and alpha-fodrin were observed in the cytoplasm of stromal and epithelial cells in decidua using immunohistochemistry. No significant differences were observed in staining patterns. Western blot analysis showed no significant differences in expression of m-calpain, calpastatin and alpha-fodrin, whereas mu-calpain was significantly higher and integrin beta3 was lower in subject. The results suggest that cleavage of integrin beta3 by mu-calpain may have an adverse effect on the mechanism of early pregnancy.