Gordoniajinhuaensis sp. nov., a novel actinobacterium, isolated from a VBNC (viable but non-culturable) state in pharmaceutical wastewater

• Published in: Antonie van Leeuwenhoek Vol. 106; no. 2; pp. 347 - 356
• Main Authors: Li, Shan-Hui, Jin, Yi, Cheng, Juan, Park, Dong-Jin, Kim, Chang-Jin, Hozzein, Wael N., Wadaan, Mohammed A. M., Shu, Wen-Sheng, Ding, Lin-Xian, Li, Wen-Jun
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.08.2014; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Genomics; Gram-positive bacteria; Life Sciences; Lipids; Medical Microbiology; Medical wastes; Microbiology; Original Paper; Pharmaceutical industry wastes; Pharmaceuticals; Plant Sciences; Sodium chloride; Soil Science & Conservation; Taxonomy; Rpf; VBNC; Pharmaceutical wastewater; Polyphasic taxonomy; sp. nov
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-014-0207-3

A Gram-stain positive, aerobic, non-motile and rod-shaped actinobacterial strain, designated as ZYR 51 T , was isolated from pharmaceutical wastewater in Jinhua city, Zhejiang province, Eastern China. Isolation was aided by using a resuscitation-promoting factor, suggesting the strain was recovered from a viable but non-culturable state. Strain ZYR 51 T was characterized by a polyphasic taxonomic approach. Growth was found to occur at 10–45 °C, pH 6.0–10.0 and 0–9 % NaCl (w/v). Based on the 16S rRNA gene sequence, phylogenetic analysis clearly demonstrated that strain ZYR 51 T belongs to the genus Gordonia and showed low level similarities (below 97 %) with all other members of this genus. The strain was found to possess meso -diaminopimelic acid ( meso -DAP), along with MK-9(H 2 ) as the predominant menaquonine. Mycolic acids were found to be present. C 16:0 (34.9 %), 10-methyl C 18:0 (30.3 %), iso-C 18:0 (8.2 %), and summed feature 3 (C 16:1 ω6c and/or C 16:1 ω7c as define by MIDI; 18.8 %) were identified as the major cellular fatty acids. The polar lipid profile of strain ZYR 51 T was found to consist of diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and some unknown lipids. The genomic DNA G+C content of strain ZYR 51 T was determined to be 67.7 mol%. The combined genotypic and phenotypic data showed that the strain represents a novel species of the genus Gordonia , for which the name Gordonia jinhuaensis sp. nov. is proposed, with the type strain is ZYR 51 T (=CGMCC 1.12827 T  = NBRL B-59111 T  = NBRC 110001 T ).