加味腎氣丸이 수컷 노화 생쥐의 생식기능에 미치는 영향

• Published in: 동의생리병리학회지 Vol. 31; no. 6; pp. 367 - 373
• Main Authors: 박선영(Sun Young Park), 안상현(Sang Hyun Ahn), 김호현(Ho Hyun Kim)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.12.2017
• Subjects: 한의학; Reproductive function; DJ-1; HDAC3; Gami-shinkiwhan(GS); 17β-HSD; Late-onset hypogonadism
• ISSN: 1738-7698; 2288-2529
• DOI: 10.15188/kjopp.2017.12.31.6.367

This study was performed using histochemical and immunohistochemical methods to investigate the effect of Gami-Shinkiwhan(GS) on reproductive function of male aged mice. 8-weeks-old ICR mice were used as control group, without any treatment, and 15-month-old ICR mice were used as aging elicited group(AE) and Gami-Shinkiwhan treatment group(GS). AE group didn’t restrict diets and drinking for 6 months without any treatment. GS was administered 0.56g/kg/day for 6 months. Compared with AE group, the cell division of sertoli cells, spermatids, and spermatogonial cells was increased and the apoptosis of sertoli cells was decreased on GS group. Androgen receptor positive reaction and 17β-HSD positive reaction were significantly increased in the GS group compared to AE group. In addition, the DJ-1 positive reaction was significantly increased and the HDAC3 positive response was significantly decreased in the GS group compared with AE group. Based on the above results, GS prevented the apoptosis of sertoli cells in the tubules, and increased the production of sertoli cells, spermatozoa and testosterone. Based on this, it is thought that it improves male reproductive dysfunction caused by late-onset hypogonadism. KCI Citation Count: 0