풍미소스류 제조를 위한 간장박 효소가수분해물의 최적화

• Published in: Han'guk Sikp'um Yŏngyang Kwahakhoe chi Vol. 46; no. 12; pp. 1517 - 1522
• Main Authors: 차용준(Yong-Jun Cha), 왕문봉(Wenfeng Wang)
• Format: Journal Article
• Language: Korean
• Published: 한국식품영양과학회 01.12.2017
• Subjects: 식품과학; by-product; enzymatic hydrolysate; response surface methodology; soy sauce residue
• ISSN: 1226-3311; 2288-5978
• DOI: 10.3746/jkfn.2017.46.12.1517

양조간장 가공부산물인 간장박을 부가성이 높은 소재로 재활용하기 위해 단백질 분해효소를 이용하여 효소가수분해물의 최적 조건을 규명하였다. 반응표면분석법을 통하여 얻어진 가수분해율(%)은 37.789+2.833[S]+2.811[E/S]+ 1.895[S]2+3.733[S][Time]과 같은 식을 얻을 수 있었다. 그러나 반응 모형식에서 얻어진 정상점(stationary point)은 안장점(saddle point)을 나타내고 있어, 능선분석(ridge analysis)을 통하여 반경(radius) 1.0에서의 예측치는 43.83 ±(1.59)2%의 가수분해율을 나타내었다. 이 조건 값에서 실제 실험을 한 결과 41.86±1.30%의 값을 얻어 예측치보다는 약간 낮은 가수분해율을 얻었다. 이러한 최적의 가수분해 조건은 기질농도=8.79%(w/v; 간장박 무게/증류수), 기질에 대한 효소농도[E/S]=0.43%(w/v, FlavourzymeTM 500 MG :ProtamexⓇ=1:1 ratio), 반응시간[Time]=4.43시간으로 나타났다. 간장박의 유리아미노산 함량은 2,764.51 mg%(건물량 기준: 4,137.87 mg%)에서 효소가수분해 후에는 570.86 mg%(건물량 기준: 23,205.77 mg%)로 건물량 기준으로 5.6배가 증가하였다. This study was performed to determine the optimal hydrolysis conditions for production of soy flavoring from soy sauce residue (SSR) as a by-product of soy sauce with commercial proteases. Using three variables (substrate concentration, enzyme/substrate ratio, reaction time), response surface methodology (RSM) based on a five level central composite design was applied to evaluate the degree of hydrolysis as the dependent variables. A model equation obtained from RSM showed an R-square value of 0.81 with a lack of fit of 0.10 (P<0.05). Optimal hydrolysis conditions for making SSR hydrolysate were obtained as follows: 8.79% (w/v) substrate concentration (pH adjusted to 7.0), 2 h reaction time with 0.4% (v/w) of AlcalaseTM 2.4 L, followed by 0.43% (w/w) Protamex® plus FlavourzymeTM 500 MG (1:1 ratio), and a reaction time of 4.43 h. The hydrolysis ratio obtained under these optimal conditions was 41.86%, which was lower than the predicted value (43.83%). The total amount of free amino acids in the hydrolysate of SSR was 5.6 times higher than that of SSR on a dry basis. KCI Citation Count: 5