도파민 유도성 SH-SY5Y 세포독성에 대한 遠志의 방어기전 연구

• Published in: 동의생리병리학회지 Vol. 18; no. 2; pp. 544 - 552
• Main Authors: 이지용(Ji Yong Lee), 박재현(Jae Hyeon Park), 김경렬(Kyung Yeol Kim), 김태헌(Tae Heon Kim), 강형원(Hyung Won Kang), 류영수(Young Su Lyu)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.04.2004
• Subjects: 한의학
• ISSN: 1738-7698; 2288-2529

In oriental medicine, Radix Polygalae(RP) has been to treat tremors et al. But the mechanism how to decrease tremors was not known. The purpose of this study was to investigate the effect of RP on neurodegenerative disease. We used RP to execute the study of this defense mechanism on dopamine-induced cell death in human SH-SY5Y dopaminergic neuroblastoma cells. MTT assay was used to know the cytotoxicity of dopamine and the defense mechanism. As a result of this experiment, dopamine had cytotoxicity in human SH-SY5Y cells, but when it treated with RP, the cell survival rate increased. This suppressed the cell apoptosis, activation of caspase-3 protease, production of ROS, and repair of membrane potential change. In conclusion, RP has the protective effect on dopamine-induced cell death in human SH-SY5Y dopaminergic neuroblastoma cells, so this could be an effective agent on the neurodegenerative disease like Parkinsonism. KCI Citation Count: 0