U-937 세포에서 導赤承氣湯 추출물 중 부탄올 분획에 의한 Apoptosis 유도

• Published in: 동의생리병리학회지 Vol. 20; no. 3; pp. 590 - 595
• Main Authors: 박평범(Pyeong Beom Park), 정한솔(Han Sol Jeong), 김호(Ho Kim), 진재호(Jae Ho Jin), 정상훈(Sang Hun Jeong), 한웅(Ung Han), 이문원(Moon Won Lee), 이광규(Kwang Gyu Lee)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.06.2006
• Subjects: 한의학
• ISSN: 1738-7698; 2288-2529

To investigate the anti-cancer effects of n-butanol fraction of DoJeokSeungKi-Tang extracts(nBFD) in U-937 cells. MTT assay was used to determine U-937 cells proliferation. Flow cytometry was used to detect apoptosis. Bcl-xl anti-apoptotic protein and caspase-3, p53 pro-apoptotic protein were examined by Western blot analysis. nBFD inhibited the proliferation of U-937 cells in a dose-dependent manner. The cells treated with nBFD showed a typical apoptotic process by increasing sub-G1 peak. nBFD reduced uptake of 3,3`dihexyloxacarbocyanine iodide(DiOC6) a fluorochrome which incorporates into cells dependent upon their mitochondrial transmembrane potential(ΔΨm). nBFD induced in U-937 cells apoptosis mainly via increasing sub-G1 peak, regulation of Bcl-xl, caspase-3 and p53 protein. KCI Citation Count: 0