夜關門이 토끼의 혈관과 음경해면체 수축에 미치는 영향

• Published in: 동의생리병리학회지 Vol. 27; no. 6; pp. 809 - 817
• Main Authors: 박선영(Sun Young Park), 김호현(Ho Hyun Kim)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.12.2013
• Subjects: 한의학; nitric oxide; Lespedezea cuneata; arterial relaxation; corpus cavernosum
• ISSN: 1738-7698; 2288-2529
• DOI: 10.15188/kjopp.2013.12.27.6.809

The aim of this study was to evaluate the mechanism of vasodilation of Lespedezea cuneata(LC) in rabbit common carotid artery and cavernosal smooth muscle. LC relaxed arterial strips precontracted with norepinephrine and cavernosal strips precontracted with phenylephrine. The arterial relaxation effects of LC was endothelium-dependent. Nω-nitro-L-arginine(L-NNA), NOS inhibitor, methylene blue(MB), cGMP inhibitor, indomethacin(IM), cyclo-oxygenase inhibitor and tetraethylammonium chloride(TEA), KCa-channel blocker attenuate the relaxation responses of LC in arterial strips. In Ca2+-free krebs-ringer solution, pretreatment of LC extract significantly reduced the contraction induced by addition Ca2+. L-NNA reduced LC extract-induced relaxation in cavernosal strips, but IM, TEA and MB didn't affect LC extract-induced relaxation. When LC extract was applicated on human umbilical vein endothelial cell, the nitric oxide concentration was increased. We conclude that in rabbit common carotid artery, LC may suppress influx of extra-cellular Ca2+ through the release of endothelium derived relaxing factor including nitric oxide, prostacyclin, endothelium derived hyperpolarizing factor. And LC exerts a relaxing effect on corpus cavernosum through activating the NO. KCI Citation Count: 2