Anti - apoptotic Effect of Bojungbangam - tang Ethanol Extract on Cisplatin - Induced Apoptosis in Rat Mesangial Cells

• Published in: 동의생리병리학회지 Vol. 20; no. 6; pp. 1664 - 1671
• Main Authors: Nam Su Kim, Sung Min Ju, Young Dal Kwon, Byung Cheul Shin, Kyoo Seok Ahn, Sung Hoon Kim, Yung Sun Song, Byung Hun Jeon
• Format: Journal Article
• Language: English
• Published: 한의병리학회 01.12.2006
• Subjects: 한의학
• ISSN: 1738-7698; 2288-2529

Cisplatin is a anti-neoplastic agent which is commonly used for the treatment of solid tumor. Cisplatin activates multiple signal transduction pathways involved in the stress-induced apoptosis in a variety of cell types. Cytotoxicity of cisplatin was detected in rat mesangial cells and the value of IC50 is about 20 μM. The treatment of cisplatin to rat mesangial cells showed the apoptotic bodies and DNA fragmentation. The activation of caspase-3, -8, and -9 and proteolytic cleavage of PARP were observed in the rat mesangial cells treated time-dependently with cisplatin. The activation of ERK, p38 and JNK was also observed in the apoptosis induced by cisplatin in rat mesangial cells. The ethanol extract of Bojungbangam-tang (EBJT), a new herbal prescription composed of nine crude drugs, inhibited cisplatin-induced apoptosis in rat mesangial cells. EBJT reduced sub-G1 peak (apoptotic peak) in cisplatin-treated rat mesangial cells. The cisplatin-induced ERK and JNK activation in rat mesangial cells were blocked by EBJT, but EBJT had no effect on p38 activation. Taken together, these results can suggest that EBJT prevents cisplatin-induced apoptotic cell death in rat mesangial cells through inhibition of ERK and JNK activation. KCI Citation Count: 1