Biological activity of SE-10, granulated powder of Sasa senanensis Rehder leaf extract

• Published in: In vivo (Athens) Vol. 26; no. 3; p. 411
• Main Authors: Sakagami, Hiroshi, Matsuta, Tomohiko, Satoh, Kazue, Ohtsuki, Sumiko, Shimada, Chiyako, Kanamoto, Taisei, Terakubo, Shigemi, Nakashima, Hideki, Morita, Yurika, Ohkubo, Atsuko, Tsuda, Tadashi, Sunaga, Katsuyoshi, Maki, Jun, Sugiura, Tomoko, Kitajima, Madoka, Oizumi, Hiroshi, Oizumi, Takaaki
• Format: Journal Article
• Language: English
• Published: Greece 01.05.2012
• Subjects: Anti-HIV Agents - pharmacology; Cell Survival - drug effects; Cell Survival - radiation effects; Cells, Cultured; Free Radical Scavengers - pharmacology; HIV-1 - drug effects; Humans; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Radiation-Protective Agents - pharmacology; Sasa - chemistry; Ultraviolet Rays
• ISSN: 1791-7549; 1791-7549

We have previously reported that alkaline extract of Sasa senanensis leaves (SE) showed potent anti-HIV, anti-UV and radical scavenging activity. In the present study, we investigated the biological activities of SE-10, a granulated powder of SE supplemented with lactose, lactitol, trehalose and tea extract. Cell viability of mock-infected, HIV-infected, and UV-irradiated cells was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Scavenging activity of superoxide anion and hydroxyl radicals was determined by electron-spin resonance spectroscopy. Cytochrome P-450 (CYP)3A4 activity was measured by β-hydroxylation of testosterone in human recombinant CYP3A4. SE-10 had slightly higher anti-HIV and anti-UV activities, but slightly lower radical-scavenging and CYP3A4-inhibitory activities, as compared with SE. The present study demonstrates that the biological activities of SE were well preserved during the manufacturing process of SE-10.