Comparative study of biological activity of three commercial products of Sasa senanensis Rehder leaf extract

• Published in: In vivo (Athens) Vol. 26; no. 2; p. 259
• Main Authors: Sakagami, Hiroshi, Iwamoto, Shoko, Matsuta, Tomohiko, Satoh, Kazue, Shimada, Chiyako, Kanamoto, Taisei, Terakubo, Shigemi, Nakashima, Hideki, Morita, Yurika, Ohkubo, Atsuko, Tsuda, Tadashi, Sunaga, Katsuyoshi, Kitajima, Madoka, Oizumi, Hiroshi, Oizumi, Takaaki
• Format: Journal Article
• Language: English
• Published: Greece 01.03.2012
• Subjects: Anti-HIV Agents - isolation & purification; Anti-HIV Agents - pharmacology; Anti-HIV Agents - toxicity; Carcinoma, Squamous Cell - pathology; Cell Line, Tumor - drug effects; Cell Line, Tumor - radiation effects; Cell Line, Tumor - virology; Cell Survival; Chlorophyllides - analysis; Chlorophyllides - pharmacology; Cytochrome P-450 CYP3A; Cytochrome P-450 CYP3A Inhibitors; Drug Combinations; Electron Spin Resonance Spectroscopy; Enzyme Inhibitors - isolation & purification; Enzyme Inhibitors - pharmacology; Enzyme Inhibitors - toxicity; Free Radical Scavengers - isolation & purification; Free Radical Scavengers - pharmacology; Free Radical Scavengers - toxicity; HIV-1; Human T-lymphotropic virus 1; Humans; Lignin - pharmacology; Lignin - toxicity; Mouth Neoplasms - pathology; Nonprescription Drugs; Panax - chemistry; Pinus - chemistry; Plant Extracts - pharmacology; Plant Extracts - toxicity; Plant Leaves - chemistry; Radiation-Protective Agents - isolation & purification; Radiation-Protective Agents - pharmacology; Radiation-Protective Agents - toxicity; Recombinant Proteins - antagonists & inhibitors; Sasa - chemistry; T-Lymphocytes - drug effects; T-Lymphocytes - virology; Ultraviolet Rays
• ISSN: 1791-7549; 1791-7549

We have previously reported that alkaline extract of Sasa senanensis leaves (SE) has several biological activities characteristic of lignin-carbohydrate complex (LCC). In the present study, we compared the biological activity of three commercially available products of SE (products A, B and C). Cell viability of mock-infected, HIV-infected, UV-irradiated cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Radical intensity was determined by electron spin resonance spectroscopy. Cytochrome P-450 (CYP)3A4 activity was measured by β-hydroxylation of testosterone in human recombinant CYP3A4. Product A is a pure SE that contains Fe(II)-chlorophyllin, whereas products B and C contain Cu(II)-chlorophyllin and less LCC. Product C is supplemented with ginseng and pine (Pinus densiflora) leaf extracts. Product A exhibited 5-fold higher anti-HIV, 4-fold higher anti-UV, 5-fold higher hydroxyl radical-scavenging, and 3-fold lower CYP3A4 inhibitory activities as compared to those of product B, and 5-fold higher, 1.5-fold higher, comparable, and 7-fold lower activities, respectively, as compared to those of product C. The present study demonstrates for the first time the superiority of product A over products B and C, suggesting the beneficial role of LCC and Fe(II)-chlorophyllin.