DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex

• Published in: Molecules and cells Vol. 39; no. 9; pp. 699 - 704
• Main Authors: Jang, Soo Hwa, Kim, Ah-Ram, Park, Neung-Hwa, Park, Jeong Woo, Han, In-Seob
• Format: Journal Article
• Language: English
• Published: United States Korean Society for Molecular and Cellular Biology 01.09.2016; 한국분자세포생물학회
• Subjects: Animals; CDC2 Protein Kinase; Cell Proliferation - physiology; Cyclin B1 - genetics; Cyclin B1 - metabolism; Cyclin B1 - physiology; Cyclin-Dependent Kinases - genetics; Cyclin-Dependent Kinases - metabolism; Cyclin-Dependent Kinases - physiology; G2 Phase Cell Cycle Checkpoints - physiology; Gene Knockdown Techniques; GTP-Binding Proteins - genetics; GTP-Binding Proteins - metabolism; GTP-Binding Proteins - physiology; HeLa Cells; Heterografts; Humans; Male; Mice; Mice, Nude; Mitosis - physiology; 생물학; DRG2; cyclin B/Cdk1 complex; G2/M check point; p21
• ISSN: 1016-8478; 0219-1032
• DOI: 10.14348/molcells.2016.0149

Developmentally regulated GTP-binding protein 2 (DRG2) plays an important role in cell growth. Here we explored the linkage between DRG2 and G2/M phase checkpoint function in cell cycle progression. We observed that knockdown of DRG2 in HeLa cells affected growth in a wound-healing assay, and tumorigenicity in nude mice xenografts. Flow cytometry assays and [(3)H] incorporation assays indicated that G2/M phase arrest was responsible for the decreased proliferation of these cells. Knockdown of DRG2 elicited down-regulation of the major mitotic promoting factor, the cyclin B1/Cdk1 complex, but up-regulation of the cell cycle arresting proteins, Wee1, Myt1, and p21. These findings identify a novel role of DRG2 in G2/M progression.