원적외선 처리된 대마종자유와 올리브유의 보충이 콜린 결핍 식이로 유도된 비알코올성 지방간염 마우스에 미치는 영향

• Published in: Han'guk Sikp'um Yŏngyang Kwahakhoe chi Vol. 53; no. 12; pp. 1227 - 1238
• Main Authors: 신미래(Mi-Rae Shin), 정성미(Sung Mi Chung), 김민주(Min Ju Kim), 안희연(Hui Yeon An), 정일하(Il-Ha Jeong), 이우락(Woo Rak Lee), 노성수(Seong-Soo Roh)
• Format: Journal Article
• Language: Korean
• Published: 한국식품영양과학회 2024
• Subjects: 식품과학; olive oil; hemp seed oil; high-fat diet; non-alcoholic steatohepatitis
• ISSN: 1226-3311; 2288-5978
• DOI: 10.3746/jkfn.2024.53.12.1227

이 연구는 원적외선 처리된 대마종자유와 올리브유 혼합물(FM)이 콜린 결핍 고지방 식이(CDHFD)로 유도된 비알코올성 지방간염(NASH) 모델에서 간의 지방 축적에 미치는 영향을 조사하였다. 실험 결과, CDHFD 대조군의 상대적 간 지방 무게가 유의하게 증가했지만, FM 처리군에서는 극적으로 감소하였다. 특히, FM 1 mL/kg/d로 투여한 처리군은 간 지방 축적, 염증 단백질, 지방세포 크기의 감소에 효과적이었다. 결론적으로, 원적외선 처리된 대마종자유와 올리브유 혼합물은 산화 스트레스 억제, 염증 완화, 지질 관련 인자들의 억제를 통해 간조직 내 지질 축적을 개선할 수 있음을 증명하였다. Non-alcoholic steatohepatitis (NASH) is a chronic liver condition seen across the world. It is characterized by fat accumulation in hepatocytes, hepatic inflammation, fibrosis, and it elevates the risk of cirrhosis. The purpose of this study was to investigate whether supplementation with a far infrared-processed hemp seed oil and olive oil mixture (FM) alters hepatic lipid accumulation in a choline-deficient high-fat diet (CDHFD)-induced NASH model. Male C57BL/6 mice were fed CDHFD for 14 weeks, and drug treatments (FML; FM low dose 1 mL/kg, FMH; FM high dose 2 mL/kg) were administered for 4 weeks, starting from week 11. While a high-fat diet (HFD) caused significant weight gain within 3 weeks, CDHFD feeding resulted in a slower rate of weight increase, with noticeable differences emerging only after 8 weeks. Notably, there was no significant difference in body weight between the CDHFD-Control group and the FM treatment group throughout the experiment. Relative fat weight (%) based on fat and body weight increased significantly in both the HFD-Control and CDHFD-Control groups. However, it was dramatically reduced following FM treatment. Specifically, FML treatment produced a 13.96% greater reduction in fat weight compared to the FMH treatment. Furthermore, FML therapy effectively suppressed lipid-related factors and inflammatory proteins in both serum and liver tissue. Additionally, FML therapy was more effective than FMH in attenuating intrahepatic lipid accumulation and decreasing adipocyte size. In conclusion, our results suggest that FML may be a promising candidate for the treatment and prevention of NASH. KCI Citation Count: 0