Osteogenesis evaluation of duck’s feetderived collagen/hydroxyapatite sponges immersed in dexamethasone

• Published in: Biomaterials research pp. 10 - 16
• Main Authors: 국연지, 이대훈, 송정은, Nirmalya Tripathy, 전유신, 전하얀, Joaquim M. Oliveira, Rui L. Reis, 강길선
• Format: Journal Article
• Language: English
• Published: 한국생체재료학회 01.03.2017
• Subjects: 의공학
• ISSN: 1226-4601; 2055-7124
• DOI: 10.1186/s40824-017-0088-4

Background: The aim of this study was to investigate the osteogenesis effects of DC and DC/HAp sponge immersed in without and with dexamethasone. Methods: The experimental groups in this study were DC and DC/HAp sponge immersed in without dexamethasone (Dex(−)DC and Dex(−)-DC/HAp group) and with dexamethasone (Dex(+)-DC and Dex(+)-DC/HAp group). We characterized DC and DC/HAp sponge using compressive strength, scanning electron microscopy (SEM). Also, osteogenic differentiation of BMSCs on sponge (Dex(−)DC, Dex(−)-DC/HAp, Dex(+)-DC and Dex(+)-DC/HAp group) was assessed by SEM, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assay, alkaline phosphatase (ALP) activity assay and reverse transcription-PCR (RT-PCR). Results: In this study, we assessed osteogenic differentiation of BMSCs on Duck’s feet-derived collagen (DC)/HAp sponge immersed with dexamethasone Dex(+)-DC/HAp. These results showed that Dex(+)-DC/HAp group increased cell proliferation and osteogenic differentiation of BMSCs during 28 days. Conclusion: From these results, Dex(+)-DC/HAp can be envisioned as a potential biomaterial for bone regeneration applications. KCI Citation Count: 2