인체 모유두세포의 증식에 미치는 갈근 에탄올추출물의 효과

• Published in: 동의생리병리학회지 Vol. 31; no. 3; pp. 167 - 172
• Main Authors: 박설아(Seol A Park), 고경숙(Kyoung Sook Ko), 인명희(Myoung Hee In), 문연자(Yeun Ja Mun), 우원홍(Won Hong Woo)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2017
• Subjects: 한의학; Puerariae Radix; Akt; cell proliferation; HHDPCs; ERK
• ISSN: 1738-7698; 2288-2529; 2283-2529
• DOI: 10.15188/kjopp.2017.06.31.3.167

In this study, we investigated the effect of Puerariae Radix ethanol extracts (EPR). The effect of the EPR on proliferation of human hair dermal papilla cells(HHDPCs) by MTT assay and observed Expression of mechanisms that regulate cell proliferation extracellular signal-regulated kinase(ERK) and Akt by western blot. The results showed EPR increased the proliferation of HHDPCs and up-regulation phosphorylation of ERK and Akt. ERK and Akt increased by EPR inhibited phosphorylation by PD98059 (ERK inhibitor) and LY294002 (Akt inhibitor), and cell proliferation was also inhibited. These results suggested EPR increases the proliferation of HHDPCs through phosphorylation of ERK and Akt, and therefore is a beneficial effect for the alopecia treatment.