삼채 뿌리의 항산화 및 수명연장 효과

• Published in: 생약학회지 Vol. 46; no. 3; pp. 234 - 242
• Main Authors: 전현일(Hyun Il Jun), 기별희(Byeolhui Ki), 이성현(Sung Hyen Lee), 김유석(You Suk Kim), 한순천(Sooncheon Han), 김대근(Dae Keun Kim), 이은별(Eun Byeol Lee), 김준형(Jun Hyeong Kim), 양재헌(Jae Heon Yang), 김영수(Young Soo Kim)
• Format: Journal Article
• Language: Korean
• Published: 한국생약학회 2015
• Subjects: 약학; Caenorhabditis elegans; Antioxidant; Lifespan; Allium hookeri
• ISSN: 0253-3073; 2288-9299

Allium hookeri (Liliaceae), native to India is widely cultivated in Korea lately as a medicinal plant. This study was performed to evaluate the antioxidant and lifespan extending effects of the root of A. hookeri. Methanol extract from the root of A. hookeri was successively partitioned as methylene chloride, ethyl acetate, n-butanol and H2O soluble fractions. Antioxidant effects of the fractions were measured by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH), and superoxide quenching activities. Lifespan extending effects of the fractions were investigated using Caenorhabditis elegans model system. In addition, stress resistant, superoxide dismutase (SOD) and catalase activities, and intracellular reactive oxygen species (ROS) levels were also studied. Furthermore, we had investigated aging-related factors such as reproduction, food intake, growth and movement of C. elegans to find any sample-induced significant change. Our results revealed that ethyl acetate soluble fraction from the root of A. hookeri showed the most potent antioxidant and lifespan extending effects, and this fraction elevated the most potent SOD and catalase activities of worms, and reduce intracellular ROS accumulation.