고욤(Diospyros lotus L.)잎 추출물의 항알레르기 및 항가려움 효과

• Published in: 생약학회지 Vol. 44; no. 1; pp. 60 - 69
• Main Authors: 김현수(Hyeon Soo Kim), 전인화(In Hwa Jeon), 목지예(Ji Ye Mok), 강현주(Hyun Ju Kang), 신준호(Jun Ho Shin), 박용균(Young Kyun Park), 정승일(Seung Ii Jeong), 장선일(Seon Ii Jang)
• Format: Journal Article
• Language: Korean
• Published: 한국생약학회 2013
• Subjects: 약학; Rat mast cells; Anti-pruritus; Mice; Anti-allergy; Diospyros lotus leaves
• ISSN: 0253-3073; 2288-9299

The worldwide prevalence and severity of allergic diseases including atopic and contact dermatitis has increased dramatically over the past decade, especially in developed countries. Mast cells are important effector cells in allergic reactions. The purpose of this study was undertaken to investigate the anti-allergic and anti-pruritic effects of Diospyros lotus leaf extract (DLE). DLE was prepared by extracting with distilled water. In the present study, we investigated the effect of DLE on the production of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\alpha}$) and histamine in rat peritoneal mast cells (RPMCs), and on the skin lesion, leukocyte infiltration and scratching behavior in mice. Phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187 significantly increased TNF-${\alpha}$ and IL-$1{\beta}$ production compared with media control. However, TNF-${\alpha}$ and IL-6 production increased by PMA plus A23187 treatment were significantly inhibited by DLE in a dose-dependent manner. DLE also inhibited the histamine release from RPMCs stimulated by compound 48/80, which promotes histamine release. Moreover, DLE administration had an inhibitory effects on the scratching behavior induced by pruritogen (compound 48/80, histamine) in ICR mice. Furthermore, DLE inhibited the skin lesions, inflammatory and mast cells in hairless mice sensitized by 2,4-dinitrofluorobenzene (DNFB). DLE administration reduced the IL-4 and IgE production induced by DNFB sensitization in hairless mice. These results suggest that DLE has a potential use as a herb medicine for treatment against allergy and pruritus-related disease.