감초 물추출물의 멜라닌 형성 억제효과

• Published in: 동의생리병리학회지 Vol. 16; no. 6; pp. 1230 - 1235
• Main Authors: 문연자(Yeun Ja Mun), 김진(Jin Kim), 임난영(Nan Young Lim), 이승연(Seung Yon Lee), 곽섭(Gwak Seop), 황충연(Chung Yeon Hwang), 우원홍(Won Hong Woo)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2002
• Subjects: 한의학; melanogenesis; Glycyrrhizae Radix; tyrosinase; DOP Achrome tautomerase
• ISSN: 1738-7698; 2288-2529; 2283-2529

This study was conducted to evaluate the effects of Glycyrrhizae Radix water extract, known as depigmenting agent, on melanin biosynthesis in cellular level. The inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis was identified by mushroom tyrosinase assay, To determine whether Glycyrrhizae Radix water extract suppress melanin synthesis in cellular level, B16 mouse melanoma cells were cultured in the presence of different concentrations of Glycyrrhizae Radix water extract. The maximum concentration of Glycyrrhizae Radix water extract that was not inhibitory to growth of the cells was 2 mg/ml. At that concentration, melanin synthesis was significantly inhibited without cytotoxicity after 5 days, compared with untreated cells. The treatment with Glycyrrhizae Radix water extract reduced tyrosinase and DOPAchrome tautomerase activity in a dose-dependent manner. These results suggest that the inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis is due to the suppression of tyrosinase and DOPAchrome tautomerase activity.