B세포의 증식에 있어 B-1 임파구와 B-2 임파구의 차이점에 대한 연구

• Published in: Immune network Vol. 4; no. 3; pp. 155 - 160
• Main Authors: 여승근(Seung Geun Yeo), 조중생(Joong Saeng Cho), 박동춘(Dong Choon Park), Thomas L. Rothstein
• Format: Journal Article
• Language: Korean
• Published: 대한면역학회 2004
• Subjects: 면역학; splenic B-2 cell; immunoglobulin; proliferation; Peritoneal B-1 cell
• ISSN: 1598-2629; 2092-6685

Background: B-1 cells differ from conventional B-2 cells both phenotypically and functionally. The aim of this study was to investigate the difference between peritoneal B-1 cells and splenic B-2 cells in proliferation. Methods: We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by enzymelinked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay. Results: Spontaneous Immunoglobulin M production occurred in peritoneal B-1 cells but not in splenic B-2 cells. LPS stimulated peritoneal B-1 cells secreted IgM at day 1, but splenic B-2 cells at day 2. In thymidine incorporation, peritoneal B-1 cells entered actively S phase after 24hours LPS-stimulation but splenic B-2 cells entered actively S phase after 48 hours. Conclusion: IgM secretion and S phase entering occurred early in peritoneal B-1 cells compared to splenic B-2 cells.