한국산 중국산 홍화자의 Nrf-2 매개 항산화 효과

• Published in: 동의생리병리학회지 Vol. 27; no. 6; pp. 745 - 751
• Main Authors: 신현종(Hyun Jong Shin), 진재호(Jae Ho Jin), 이광규(Kwang Gyu Lee), 이창현(Chang Hyun Lee), 이상룡(Sang Ryong Lee), 하기태(Ki Tae Ha), 주명수(Myungsoo Joo), 정한솔(Han Sol Jeong)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2013
• Subjects: 한의학; Anti-oxidant response element; Reactive oxygen species; Safflower seeds; Nrf-2
• ISSN: 1738-7698; 2288-2529; 2283-2529
• DOI: 10.15188/kjopp.2013.12.27.6.745

Safflower (Carthamus tinctorius L.) seeds have been used in Korea and China for promoting bone formation and protection. This study was designed to examine the Nrf-2 mediated anti-oxidative effects of Korean and Chinese safflower seeds. Water and ethanol extracts of safflower seeds were treated to RAW 264.7 cells. Nrf-2 transcriptional activity was measured by reporter gene assay and western blot analysis. Semi-quantitive RT-PCR analysis was adopted to measure Nrf-2 dependent gene expressions. Water extracts of safflower seeds have strongly induced the activation of Nrf-2 transcription than ethanol extracts. Especially, water extracts of Korean safflower seeds has more strongly increased the expression of nuclear Nrf-2. Water extracts of Korea and China safflower seeds have also increased the expression of Nrf-2-dependent genes such as GCLC, NQO-1 and HO-1 in RAW 264.7 cells. However, all kinds of safflower seeds extracts did not increase intracellular ROS production. These results demonstrate that the antioxidant effects of safflower seeds are not related with ROS production, rather it is mediated by the direct activation of Nrf-2.