엉겅퀴 추출물의 기능 성분 분석 및 TGF-beta에 의한 간 성상 세포 활성 억제 효과

• Published in: 생약학회지 Vol. 44; no. 2; pp. 110 - 117
• Main Authors: 김선영(Seon Young Kim), 김상준(Sang Jun Kim), 최영지(Young Ji Choi), 유강열(Kang Yeol Yu), 정창호(Chang Ho Chung), 심재석(Jae Suk Shim), 장선일(Seon Il Jang), 유동현(Dong Hyun Yu), 정승일(Seung Il Jeong)
• Format: Journal Article
• Language: Korean
• Published: 한국생약학회 2013
• Subjects: 약학; Flavonolignans; Hepatic stellate cells; Flavonoids; Cirsium japonicum
• ISSN: 0253-3073; 2288-9299

Cirsium japonicum (CJ) leaf (L) alcoholic extracts were investigated for analysis their active components (flavonoids and flavanolignans; silymarins) and inhibitory effect on transforming growth factor (TGF)-${\beta}$ induced hepatic stellate cells (HSCs, LX-2 cells) activation. The CJ root (R) extracts were also analyzed and compared with leaf extracts. Total flavonoid and polyphenol contents of the leaf extracts showed higher than those of the root extracts. The content of each flavonoid compound, which was analyzed by HPLC, in CJ-L extracts was also higher than in CJ-R extracts. The results of flavanolignans content in CJ-L and CJ-R extracts were consistent in flavonoid and polyphenol. We studied inhibitory effect of two extracts against TGF-${\beta}1$ induced HSCs activation. The CJ-L extracts significantly suppressed overexpression of profibrogenic factor, ${\alpha}$-smooth muscle actin and collagen-${\alpha}1$(I). The CJ-R extract also showed inhibitory effect on TGF-${\beta}1$ induced HSCs activation, but the efficacy was lower than in CJ-L extract. These results suggest that CJ-L may contribute to the fibrotic liver treatment.