함양에서 재배된 여주의 추출조건에 따른 생리활성

• Published in: Han'guk Sikp'um Yŏngyang Kwahakhoe chi Vol. 44; no. 11; pp. 1637 - 1644
• Main Authors: 문정한(Jeong-Han Moon), 최동원(Dong-Won Choi), 김성은(Seong-Eun Kim), 서문지훈(Ji-Hoon Seomoon), 홍수영(Su-Young Hong), 김현규(Hyun-Kyu Kim), 조계만(Kye-Man Cho), 송진(Jin Song), 강상수(Sang-Soo Kang), 김강화(Kang-Hwa Kim), 권오경(Oh-Kyung Kwon)
• Format: Journal Article
• Language: Korean
• Published: 한국식품영양과학회 2015
• Subjects: 식품과학; α-glucosidase; antioxidant; ethanol extract; Momordica charantia L; pancreatic lipase; {\alpha}$-glucosidase
• ISSN: 1226-3311; 2288-5978

To develop health-functional food ingredients for improvement of diabetes, we examined biological activities, including total contents of polyphenols and flavonoids, antioxidant activity, and inhibition of ${\alpha}$-glucosidase and pancreatic lipase activities of ethanol extracts from unripe fruit of Momordica charantia L. cultivated in Hamyang, Korea. Ethanol extracts were tested using different ethanol concentrations (0%, 30%, 50%, and 70%) and temperatures ($50^{\circ}C$ and $70^{\circ}C$). Experimental results showed that total polyphenol and flavonoid contents were achieved concentrations of 7.77 mg/g (tannic acid), 7.66 mg/g (gallic acid), and 10.66 mg/g (rutin). The hydroxyl radical scavenging, ABTS cation radical scavenging, and FRAP activities were 82.19%, 42.82%, and 0.19 under the same conditions, respectively. The highest inhibitory activities of ${\alpha}$-glucosidase and pancreatic lipase were achieved in the 70% ethanol extract at $70^{\circ}C$. These results will be useful for development of health functional foods for anti-diabetes using 70% ethanol extract from unripe fruit of bitter melon. 본 연구에서는 항당뇨 소재로 잘 알려져 있는 여주를 이용한 혈당 개선 건강기능성 원료 개발의 기초 연구로 주정 추출조건에 따른 항산화, 항당뇨, 항비만 효능을 검증하였다. 여주주정 추출물의 총 폴리페놀과 총 플라보노이드 함량, 항산화능, 탄수화물과 지방의 흡수 지연능을 평가하는 ${\alpha}$-glucosidase 및 pancreatic lipase 저해 활성은 온도 $70^{\circ}C$, 주정농도 70% 추출조건에서 가장 높게 나타났다. 본 연구 결과를 바탕으로 향후 여주의 항산화, 항당뇨, 항비만 효능 등 대사성 질환에 대한 예방 및 개선 효과를 가지는 건강기능식품을 개발하는 데 기초자료로 활용될 것으로 기대된다.