고욤(Diospyros lotus L.)잎 Ethyl Acetate 분획물의 멜라닌형성 억제 효과

• Published in: 생약학회지 Vol. 45; no. 3; pp. 220 - 226
• Main Authors: 최병민(Byung Min Choi), 전인화(In Hwa Jeon), 김상준(Sang Jun Kim), 유강열(Kang Yeol Yu), 장선일(Seon Il Jang)
• Format: Journal Article
• Language: Korean
• Published: 한국생약학회 2014
• Subjects: 약학; Ethyl acetate fraction; Anti-melanogenesis; B16 melanoma cells; Diospyros lotus leaves
• ISSN: 0253-3073; 2288-9299

Diospyros lotus has been cultivated for its edible fruits and leaves which are considered for its medicinal importance. The aim of this study was to evaluate the anti-melanogenesis of ethyl acetate (EA) fractions from D. lotus leaves in B16 cells. The order of the total polyphenol content with regard to the different solvent fractions from D. lotus leaves was EA>butanol>metahanol>chloroform>n-hexane. The major compounds of EA fraction from D. lotus leaves by HPLC analysis were myricitrin and myricetin. Cellular TYR activity and melanin content in response to treatment with 100 mg/mL of EA fraction was inhibited more strongly than group treated with arbutin. Further, EA fraction exhibited significant anti-melanogenesis effects by reducing the levels of microphthalima-associated transcription factor (MITE), inhibiting the synthesis of TYR, tyrosinase-related protein-1 (TRP-1) and TRP-2. Therefore, EA fractions from D. lotus leaves may be a good source of skin-whitening agents in the future development of medicine-based trouble skin therapy.