피카오프레토 등 복합물 중 총 폴리페놀 분석법 표준화

• Published in: Han'gug sigpum wi'saeng anjeonseong haghoeji Vol. 33; no. 1; pp. 44 - 49
• Main Authors: 허수정, 김지안, 문명희, 이성혜, 윤혜성, 홍진환, Hu, Soojung, Kim, Ji-An, Moon, Myung-Hee, Lee, Sung-Hye, Yoon, Hae-Seong, Hong, Jin-Hwan
• Format: Journal Article
• Language: Korean
• Published: 한국식품위생안전성학회 28.02.2018
• Subjects: 식품과학; Tannic acid; Health/Functional food; Total polyphenol; Analytical method
• ISSN: 1229-1153; 2465-9223
• DOI: 10.13103/JFHS.2018.33.1.44

As generic health functional food items have been expanded, this research project has been conducted to prepare a scientific and systematic standardized analytical method of relevant food item and examine the suitability of the method for health/functional foods on sale. Total polyphenol was necessary for development and verification of standardized analytical method. The method exhibited high linearity in the tannic acid calibration curve ($r^2$ > 0.999) over concentrations of $5-50{\mu}g/mL$. The limits of detection and quantitation for tannic acid were $5{\mu}g/mL$ and $15{\mu}g/mL$, respectively, while tannic acid recovery was 102.3-112.4% with standard deviations of 0.8-3.2%. To verify the accuracy of the analytical method, the labeled amounts of purchased health functional foods were monitored. The recovery for tannic acid was 105.6% of the labeled amounts. Thus, the new method was suitable for all cases. 피카오프레토 등 복합물의 개별인정 시험법인 총 폴리페놀 시험법에서 Folin-phenol 시약 선정, 발색시약인 35% 탄산나트륨의 반응시간 및 온도, 시료량 등을 비교 실험하여 총 폴리페놀에 대한 표준화된 시험법을 확립하였다. 총 폴리페놀의 표준화된 시험법의 밸리데이션을 수행한 결과 회수율은 102.3~112.4%, 정밀도의 표준편차는 0.81~3.18%였으며 검량선의 직선성은 결정계수($r^2$)가 0.999이상을 나타냈고 검출한계는 $5{\mu}g/mL$, 정량한계는 $15{\mu}g/mL$이었다. 유통제품에 대해 표준화된 총 폴리페놀 시험법의 적용성 검토를 위해 1건의 검체를 수거하여 분석한 결과, 총 폴리페놀 표시 함량 대비 105.6%로 적합하였다.