백진주의 저아밀로스 유전분석 및 분자지도 작성

• Published in: Korean journal of breeding Vol. 48; no. 3; pp. 234 - 240
• Main Authors: 이종희(Jong-Hee Lee), 조준현(Jun-Hyeon Cho), 이지윤(Ji-Yoon Lee), 손영보(Young-Bo Sohn), 한상익(Sang-Ik Han), 신동진(Dongjin Shin), 송유천(You-Chun Song), 박동수(Dong-Soo Park), 여운상(Un-Sang Yeo), 오명규(Myeong- Gyu Oh), 허연재(Yeon-Jae Hur)
• Format: Journal Article
• Language: Korean
• Published: 한국육종학회 30.09.2016; The Korean Breeding Society
• Subjects: 농학; rice; MAS; low amylose; Baegjinju; molecular map
• ISSN: 0250-3360; 2287-5174
• DOI: 10.9787/KJBS.2016.48.3.234

The amylose contents of rice determine eating quality which is one of the major traits in rice breeding program. To identify the low-amylose gene of the japonica rice cultivar Baegjinju, genetic analysis was conducted using 200 F2 population derived from a cross between the japonica cultivars, Saeilmi and Baegjinju. Individual F2 plants were classified as wild type (translucent grain) and mutant type (dull grain) based on the grain appearance of brown rice. Two hundred F2 plants were segregated into 155 wild type plants and 45 mutant type plants, which fit the 3:1 ratio (x2 = 0.667, df = 1, p = 0.414) and this result indicated the low-amylose gene of Baegjinju is a single recessive gene which controls the amylose contents. Linkage analysis was conducted to localize the low-amylose gene of Baegjinju and fine mapped within an 800-kb interval between 17.5 to 18.8Mb on short arm of chromosome 10. Co-segregated SSR marker, RM25648 was developed and it could be useful for marker-assisted selection and determination of the genetic resource related with amylose contents in rice breeding.