정향 추출물이 tert-butyl hydroperoxide로 유도된 HepG2 세포자멸사에 미치는 영향

• Published in: 大韓本草學會誌 Vol. 39; no. 6; pp. 25 - 32
• Main Authors: 안이슬, Iseul An, 김상찬, Sang Chan Kim, 박숙자, Sook Jahr Park, 이종록, Jong Rok Lee
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 30.11.2024
• Subjects: apoptosis; Clove; Nrf2; oxidative stress; tBHP; 한의학; apoptosis; tBHP; Nrf2; oxidative stress; Clove
• ISSN: 1229-1765; 2288-7199
• DOI: 10.6116/kjh.2024.39.6.25

Objective : Cloves, the dried flower buds of Syzygium aromaticum, have been used to treat various allergic disorders and relieve abdominal pain caused by chills. In this study, cytoprotective and antioxidant effects of cloves extracts (CLE) were investigated in tert-butyl hydoroperoxide (tBHP)-treated HepG2 cells. Methods : CLE was prepared by extracting 100 g of ground clove in 1 L of pretanol for 48 h. Several biomarkers that modulate cell viability and oxidative stress, including caspases and glutathione (GSH), were determined using Western blotting and commercial kits. Results : In HepG2 cells, tBHP induced oxidative stress and inhibited cell viability. However, CLE reduced oxidative stress via inhibiting the increase of reactive oxygen species (ROS) and depletion of GSH caused by tBHP. In TUNEL assay, tBHP-induced oxidative stress led apoptosis, but apoptosis was suppressed by CLE. The expression changes of apoptosis-related proteins, including AIF, procaspase-3, and PARP were also restored by CLE. Moreover, CLE increased the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and inhibited the phosphorylation of c-Jun N-terminal kinase (JNK) and p38 induced by tBHP. Conclusion : These results demonstrate the protective effect of CLE against tBHP-induced apoptosis in HepG2 cells, suggesting that the antioxidant activity of CLE, which scavenges ROS and restores GSH depletion via Nrf2 and JNK/p38 signaling pathways, contributes to cytoprotection. 1)