한방 추출물의 역류성 식도염 점막보호 효과에 대한 스크리닝

• Published in: 大韓本草學會誌 Vol. 39; no. 1; pp. 39 - 47
• Main Authors: 정일하, Il-ha Jeong, 김민주, Min Ju Kim, 신미래, Mi-rae Shin, 노성수, Seong-soo Roh
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 01.01.2024
• Subjects: COX-2; HET-1A; MUC5AC; NF-κB; Reactive Oxygen Species; 한의학; Reactive Oxygen Species; COX-2; HET-1A; MUC5AC; NF- ${\kappa}B
• ISSN: 1229-1765; 2288-7199
• DOI: 10.6116/kjh.2024.39.1.39

Objectives : This study evaluates how various traditional Korean herbal medicines assess MUC5AC expression for esophageal mucosal defense and analyzes the associated mechanisms involved in inflammation. Methods : Forty types of traditional Korean herbal medicines were assessed for in vitro antioxidant activities, and the real-time PCR method was employed to analyze MUC5AC expression under pH 4.5 conditions in human esophageal epithelial cells (HET-1A). Eight types of Korean herbal medicines were evaluated for in vitro antioxidant activities, and Reactive oxygen specise (ROS) expression was analyzed under bile salt (480 μM) and pH 5.5 conditions in human esophageal epithelial cells (HET-1A). Simulation experiments involving bile salts and acidity were conducted for one hour to assess the efficacy of four drugs, and the activities of Mitogen-activated Protein Kinase (MEK), Nuclear Factor Kappa B (NF-κB), and Cyclooxygenase-2 (COX-2) were detected through Western blot analysis. Results : Compared to the Normal group, the Control group exhibited higher ROS generation. Such increased ROS levels were significantly reduced by four extracts: Citrus Unshius Pericarpium (CUP), Cnidium officinale Rhizoma (CR), Ginseng Radix (GR), and Linderae Radix (LR). The protein expression of COX-2 decreased with the treatment of LR, CUP, and CR. Particularly, CUP and CR exhibited superior effects compared to other groups in inhibiting the phosphorylation of NF-κB. Conclusion : Based on the results obtained, we have identified drugs that inhibit oxidative stress and inflammation caused by bile acid in esophageal epithelial cells. Our future plans involve comparing and analyzing the efficacy of these herbal medicines through animal experiments.