차전초 잎의 물 추출물이 고지방식이로 비만이 유도된 C57BL/6 생쥐에서 지질대사 단백질 발현에 미치는 영향

• Published in: 大韓本草學會誌 Vol. 33; no. 3; pp. 63 - 70
• Main Authors: 김소영, So Young Kim, 정미진, Mi Jin Jeong, 김유진, Yoo Jin Kim, 이은탁, Un-tak Lee, 추성태, Sung-tae Choo, 김미려, Mi Ryeo Kim
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 31.05.2018
• Subjects: anti-obesity; Plantago asiaticae Folium; protein expression; 한의학; Plantago asiaticae Folium; protein expression; anti-obesity
• ISSN: 1229-1765; 2288-7199
• DOI: 10.6116/kjh.2018.33.3.63

Objective : Previous studies showed that water extract of Plantago asiatica (Plantaginis asiaticae Folium, PAF) significantly controlled in body weights, adipose tissue weights and blood lipid profiles in obese C57BL/6 mice. To investigate the mechanism of anti-obesity action of PAF, expressions of obesity-related proteins were identified such as p-AMPK and p-ACC in hypothalamus, UCP-1 in brown adipose tissue, p-AMPK, p-ACC, SREBP-1c, PPARγ, HMGCR and CPT-1 in liver. Method : Five-weeks old male C57BL/6 mice were divided into 5 groups; ND (normal diet + 0.9% saline), HFD (high-fat diet + 0.9% saline), PC (high-fat diet+Garcinia cambogia 500 ㎎/㎏), PAF 100 and 300 (high-fat diet + PAF 100 or 300 ㎎/㎏). PAF was treated orally for 6 weeks. The protein expression of AMPK, p-AMPK, ACC, p-ACC, PPARγ, SREBP-1c, HMGCR, CPT-1 and UCP-1 were identified by expression levels of proteins through western blot analysis. Result : The results showed that protein expressions on hypothalamic p-AMPK and p-ACC did not differ between the HFD and PAF groups. In addition, PAF did not affect the increase of UCP-1 in brown adipose tissue. The protein expression levels of hepatic p-AMPK, p-ACC and CPT-1 increased in PAF groups compared to HFD group. And those of PPARγ, SREBP-1c and HMG-CoA decreased in PAF groups compared to HFD group. Conclusion : These results suggest that the PAF administration induce weight loss via inhibition of lipid metabolism-related protein expressions in hepatic tissues. Therefore, PAF could be used as a potent material of anti-obesity products for prevention and treatment of obesity.