갈화(葛花)와 진피(陳皮) 추출물로 이루어진 복합물의 에스트로겐 활성과 파골세포 분화억제효과

• Published in: 大韓本草學會誌 Vol. 35; no. 3; pp. 1 - 8
• Main Authors: 조호성, Hosong Cho, 이보영, Boyoung Lee, 이원경, Won Kyung Lee, 이준호, Jun Ho Lee, 박동준, Dongjun Park, 최창일, Changil Choi, 진무현, Mu Hyun Jin, 노석선, Seok-seon Roh, 주영승, Young-sung Ju
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 31.05.2020
• Subjects: Anti-osteoclastogenesis; Citri Unshius Pericarpium; Estrogenic Activities; PCE17; Puerariae Flos; 한의학; Puerariae Flos; Citri Unshius Pericarpium; Anti-osteoclastogenesis; PCE17; Estrogenic Activities
• ISSN: 1229-1765; 2288-7199

Objectives: In this study, we examined the estrogenic activities and anti-osteo clastogenesis effects of PCE17, a mixture of PE (an extract of Puerariae Flos), and CE (an extract of Citri Unshius Pericarpium). Methods: The estrogenic effect of PCE17, PE and CE were examined by ER-β/ERE reporter gene assay and proliferation assay in 293 T and MCF-7 cells. The expression of estrogen-responsive gene and protein were checked by Real Time-PCR (RT-PCR) and Western blotting in MCF-7 cells. Inhibitory effect of PCE17, PE and CE on RANKL-induced osteoclast differentiation were evaluated by TRAP staining and RT-PCR in primary osteoclast precursors from rat bone marrow cells. Results: PCE17 and PE bind to ERs (estrogen receptors) and show estrogenic activities in 293T cells. They also stimulated the proliferation of MCF-7 cells and increased the expression of ER response gene, pS2. Tectorigenin, an active ingredient of PE, shows similar estrogenic activities in MCF-7 cells. PCE17 and CE inhibited RANKL-induced osteoclastogenesis in rat primary osteoclast precursor cells and down-regulated the osteoclast-specific genes of Nfatc1, Ctsk, and Acp5. Conclusions: In conclusion, PCE17 may have therapeutic potential in cases of menopause and osteoporosis.