내병성 자포니카 벼 계통 육성과 저항성 유전자 집적효과

• Published in: Korean journal of crop science Vol. 65; no. 4; pp. 314 - 326
• Main Authors: 김우재, Woo-jae Kim, 백만기, Man-kee Baek, 박현수, Hyeon-su Park, 이건미, Geon-mi Lee, 이창민, Chang-min Lee, 김석만, Seok-man Kim, 조영찬, Young-chan Cho, 서정필, Jeong-phil Seo, 정오영, O-young Jeong
• Format: Journal Article
• Language: Korean
• Published: 한국작물학회 01.12.2020
• Subjects: bacterial blight; chromosome segments; gene pyramiding; multi-resistant; rice; 농학; bacterial blight; multi-resistant; gene pyramiding; chromosome segments; rice
• ISSN: 0252-9777; 2287-8432

This study was carried out to develop a resistant variety against the K3a race of bacterial blight, Xanthomonas oryzae pv. oryzae, through expansion and pyramiding of resistance genes. To develop an elite bacterial blight-resistant cultivar, the breeding process and bacterial blight resistance reactions in advanced backcross lines (ABLs) were analyzed. ABLs21 which contain Xa3 and Xa21, were developed by double backcrossing japonica cultivar Hwanggeumnuri, which has bacterial blight resistant Xa3 gene, and indica variety IRBB21, which havs Xa21 gene, followed by disease resistance bioassay and marker-assisted selection. The resistance genes of ABLs21 were amplified by PCR with the molecular markers 9643.T4 (Xa3) and U1/I1 (Xa21). Hwanggeumnuri and IRBB3 showed resistance reactions against K1, K2, and K3 races, and a susceptible reaction against K3a, K4, and K5 races. IRBB21 showed resistance reactions against K2, K3, K3a, K4 and K5 races, and a susceptible reaction against K1 race. Hwanggeumnuri showed susceptible reactions at the seedling, tillering and adult stages (all stages), whereas ABL21-1 showed moderate resistance at the tillering stage. ABL21-1 showed stable resistance against 18 isolates of K3a race, and the lesion length was shorter than that of the donor parents. In cluster analysis, the HB4032 isolate showed the highest pathogenicity among the 18 isolates. The molecular marker polymorphisms and average substituted chromosome segment lengths of ABLs21 were 63.2 % and 86.1 cM, respectively. Insertion of the donor chromosomal segments occurred in the predicted region of the Xa21 gene of ABLs21.