OMC-2010 구성약재가 마우스의 비장세포 cytokine 생성에 미치는 영향

• Published in: 大韓本草學會誌 Vol. 27; no. 6; pp. 49 - 54
• Main Authors: 배기상, Gi Sang Bae, 김현식, Hyun Sik Kim, 박경철, Kyoung Chel Park, 최선복, Sun Bok Choi, 조일주, Ii Joo Jo, 이창혁, Chang Hyuk Lee, 서상완, Sang Wan Seo, 김종진, Jong Jin Kim, 신용국, Yong Kook Shin, 김민선, Min Sun Kim, 박규환, Kyu Hwan Park
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 30.11.2012
• Subjects: inflammation; lipopolysaccharide; LPS; OMC-2010; Rehmannia glutinosa; RG; splenocyte; 한의학; lipopolysaccharide (LPS); splenocyte; inflammation; Rehmannia glutinosa (RG); OMC-2010
• ISSN: 1229-1765; 2288-7199

Objective: We recently reported that OMC-2010 has an immuno-modulatory effects via inhibiting tumor necrosis factor (TNF)-α and interleukin (IL)-5. However, we did not find out which constituents play an important role in immuno-modulatory effect of OMC-2010, Thus, this study was performed to estimate the effects of constituents of OMC-2010 on cytokine production in mouse spleen cells, then ultimately reach to find out effective constituents regulating splenic cytokine production. Methods: Mouse spleen cells were pre-treated with water and ethanol extract of constituents of OMC-2010 such as Rehmannia glutinosa (RG), Pinellia ternata (PT), Citrus unshiu Markovich (CUM), Glycyrrhiza uralensis (GU), Platycodon grandiflorum (PG), Schisandra chinensis (SC). After 1 h, the cells were stimulated with lipopolysaccharide (LPS, 1 ㎍/ml) for 48 h. Then the cells were harvested for real-time reverse transcription polymerase chain reaction to detect cytokine productions. Results: The water extract of RG extract significantly inhibited the LPS-induced inTNF-α and IL-5 mRNA expressions, but the water extract of PT, CUM, GU, PG, and SC did not. The ethanol extract of RG, PT, and SC significantly inhibited the LPS-induced TNF-α, and IL-5 mRNA expressions, but the ethanol extract of CUM, GU, and PG did not. Conclusions: Theses results could suggest that the water extract of RG and the ethanol extract of RG, PT, and SC inhibited the expression of TNF-α and IL-5, which means that the possible candidate of OMC-2010 water extract`s action might be RG, and ethanol extract`s action might be RG, PR, and SC.