신경병증성 통증모델 쥐에서 리도케인의 ERK 1/2와 CREB 단백질 억제효과

• Published in: Korean journal of anesthesiology Vol. 56; no. 3; pp. 319 - 324
• Main Authors: 주진덕, Jin Deok Joo, 인장혁, Jang Hyeok In, 정홍수, Hong Soo Jung, 김용신, Yong Shin Kim, 김대우, Dae Woo Kim, 최우영, Woo Young Choi, 신은영, Eun Young Shin, 전연수, Yeon Soo Jeon
• Format: Journal Article
• Language: Korean
• Published: 대한마취통증의학회 30.03.2009
• Subjects: CREB; ERK 1/2; Intracellular cell-signaling pathway; Lidocaine; Neuropathic pain; Western blots; 마취과학
• ISSN: 2005-6419; 2005-7563

Background: In addition to causing the loss of voluntary sensory and motor function, spinal cord injury (SCI) often creates a state of central neuropathic pain. Rats given SCI display increases in the activated form of transcription factors ERK 1/2, p38 MAPK, and CREB in the spinal cord, which correspond to allodynia in a model of neuropathic pain. The current study was designed to determine if lidocaine had an effect on the development of neuropathic pain in response to SCI. Methods: Male Sprague Dawley rats were anesthetized and then received a L5-L6 spinal nerve ligation (neuropathic rats). The levels of intracellular cell-signaling protein, ERK 1/2 and CREB were then assessed by western blot analysis of samples collected from a sham operated (control) group, a neuropathic pain and normal saline (NP＋NS) group, and a neuropathic pain and 5% lidocaine (NP＋Lido) group. Results: The increased levels of ERK 1/2 and CREB protein that were observed in the neuropathic pain model were reduced by continuous infusion of 5% lidocaine. Conclusions: The current results suggest that lidocaine therapy may be an effective method of preventing and treating central neuropathic pain following SCI, and that these effects may occur via the reduced expression of ERK 1/2 and CREB in the intracellular cell-signaling pathway. (Korean J Anesthesiol 2009; 56: 319~24)