에케베리아 라우이(Echeveria laui)와 엘레강스(Echeveria elegans)의 대량증식 을 위한 조직배양 및 순화 조건 확립

• Published in: Journal of plant biotechnology Vol. 46; no. 1; pp. 22 - 31
• Main Authors: 김윤희(Youn Hee Kim), 이지영(Gee Young Lee), 김혜형(Hye Hyeong Kim), 이재홍(Jae Hong Lee), 정재홍(Jae Hong Jung), 이상덕(Sa
• Format: Journal Article
• Language: Korean
• Published: Korean Society for Plant Biotechnology 31.03.2019; (사)한국식물생명공학회; 한국식물생명공학회
• Subjects: 농학; Acclimatization; Propagation; Echeveria; Regenerated shoot; Growth regulators
• ISSN: 1229-2818; 2384-1397
• DOI: 10.5010/JPB.2019.46.1.022

The objective of this study was to investigate the suitable parts for callus induction and optimal concentrations of growth regulators contained in the medium affecting shooting and rooting Echeveria laui and Echeveria elegans for in vitro mass production. To determine the suitable plant parts for callus induction, the leaves were divided into upper, medium and bottom parts and cultured on MS medium at different concentrations with 0 ~ 2 mgL-1 NAA and 0 ~ 4 mgL-1 BA. The upper and middle parts of leaves both showed 100% callus formation rate with NAA 1 mgL-1 and BA 1 mgL-1 treatment in E. laui. The middle parts of leaves showed 83.3% callus formation rate at NAA 2 mgL-1 and BA 4 mgL-1 treatment in E. elegans. The shoot induction rate from callus was highest at NAA 0.1 mgL-1 and BA 3 mgL-1 treatment in E. laui and NAA 0.3 mgL-1 in E. elegans. In addition, the number of shoots formation was 10.4 shoots high in NAA 1 mg L-1 and BA 1 mgL-1 treatment in E. laui and 12.0 shoots in most effective NAA 1 mgL-1 and BA 0.1 mg L-1 treatment in E. elegans. In the case of acclimatization of regenerated plant, growth characteristics did not show any significant difference (35 ~ 55%) shading with respect to the different ratio of substrate mixture, and it was determined that would be appropriate considered plant height and appearance preference of E. laui and E. elegans. It was established that the optimization of culture condition was responsible for the mass propagation in vitro cultures of E. laui and E. elegans. KCI Citation Count: 1