고욤나무 잎으로부터 활성유도 분획법에 의한 α-Glucosidase 저해물질 분리 및 확인

• Published in: 생약학회지 Vol. 46; no. 2; pp. 105 - 108
• Main Authors: 곽설화(Seol Hwa Kwak), 유강열(Kang Yeol Yu), 장선일(Seon Il Jang), 김선영(Seon Young Kim), 정승일(Seung Il Jeong), 김상준(Sang Jun Kim), 김지애(Ji Ae Kim), 김다혜(Da Hye Kim)
• Format: Journal Article
• Language: Korean
• Published: 한국생약학회 2015
• Subjects: Antidiabete; Diospyros lotus; Acarbose; α-Glucosidase; Myricetin-3-O-α-L-rhamnoside; {\alpha}$-Glucosidase; Myricetin-3-O- ${\alpha}$-L-rhamnoside
• ISSN: 0253-3073; 2288-9299

To establish the anti-diabetic(α-glucosidase inhibitory) activity of D. lotus leaf extract, isolate and identify the constituents responsible for the activity. The methanolic extract of leaves was partitioned between water, n-butanol and ethyl acetate. Bio-assay guided fractionation, based on inhibition of ;${\alpha}$-glucosidase, allowed isolation and identification of the active components. Liquid chromatography/mass spectrometry(LC/MS), 1 H-NMR and 13 C-NMR spectra analyses demonstrated that the active compound was myricetin-3-O-;${\alpha}$-L-rhamnoside(1). Compound 1 demonstrated a strong inhibition on the α-glucosidase, in vitro and ;${\alpha}$-glucosidase inhibitory value was calculated as 98.08%, when that of a reference drug, acarbose was estimated as 83.03%. The present study indicates compound 1 could be considered as an ;${\alpha}$-glucosidase inhibitor and developed as an important antidiabetes agent for type II diabetes therapy.