포제에 따른 두충의 지표성분 함량분석

• Published in: 생약학회지 Vol. 46; no. 2; pp. 123 - 132
• Main Authors: 서창섭(Chang Seob Seo), 김정훈(Jung Hoon Kim), 신현규(Hyeun Kyoo Shin), 김병수(Byoung Soo Kim)
• Format: Journal Article
• Language: Korean
• Published: 한국생약학회 2015
• Subjects: Pinoresinol Diglucoside; Geniposide; Processing Method; Eucommiae Cortex; Geniposidic Acid
• ISSN: 0253-3073; 2288-9299

In this study, we carried out quantification analysis of the six marker components, geniposidic acid, chlorogenic acid, geniposide, pinoresinol diglucoside, liriodendrin, and genipin in the 70% ethanol extracts of non-processed Eucommiae Cortex and processed Eucommiae Cortex using a high-performance liquid chromatography coupled with photodiode array detector. The six components were separated on Gemini C18 column (5 μm, 4.6×250 mm) by the gradient elution with 1.0% (v/v) acetic acid in water and 1.0% (v/v) acetic acid in acetonitrile as mobile phase. The flow rate was 1.0 mL/min and the injection volume was 10 mL. The amount of geniposidic acid, chlorogenic acid, geniposide, pinoresinol diglucoside, liriodendrin, and genipin in non-processed Eucommiae Cortex were 1.31, 0.31, 0.66, 0.46, 0.46, and 0.03%, respectively, while the amount of the six compounds in non-processed Eucommiae Cortex were 0.04-0.78, 0.01-0.14%, 0.05-0.63%, 0.01-0.37%, 0.15-0.42%, and not detected, respectively. After processing treatment, the contents of three iridoids, two lingnan, and one phenylpropanoid decreased in Eucommiae Cortex.