산화적 스트레스에 대한 천마 추출물의 신경세포 보호 및 항산화 효과

• Published in: 동의생리병리학회지 Vol. 36; no. 6; pp. 209 - 212
• Main Authors: 권강범(Kang Beom Kwon), 김하림(Ha Rim Kim), 김예슬(Ye Seul Kim), 박은희(Eun Hee Park), 최한별(Han Byeol Choi), 류도곤(Do Gon Ryu)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2022
• Subjects: Anti-oxidants enzymes; Neuroprotective effects; Hydrogen peroxide; Gastrodia elata; SH-SY5Y cell
• ISSN: 1738-7698; 2288-2529; 2283-2529
• DOI: 10.15188/kjopp.2022.12.36.6.209

We recently reported that Gastrodia elata extracts (GEE) had an effects to protect against lipopolysaccharide-induced cognitive impairment in vivo model. In this study, we investigated the neuroprotective effects and the mechanism of action of GEE in hydrogen peroxide (H2O2)-induced cell death of SH-SY5Y human neuroblastoma cell. The SH-SY5Y cells were divided into five groups, including control(non-treated group), 100 μM H2O2, 100, 200, 500 ㎍/㎖ GEE+ 100 μM H2O2 groups. Pre- and co-treatment with GEE prevented cell death induced by 100 μM H2O2 for 24 h in SH-SY5Y cells. Our findings also showed that anti-oxidants enzymes (Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase) were up-regulated by 100 μM H2O2. But GEE suppressed H2O2-induced anti-oxidants enzymes decrease in a dose-dependent manner. Treatment with GEE also inhibited phosphorylation of eukaryotic initiation factor-2α (eIF-2α) and p38 by H2O2. Taken together, the neuroprotective effects of GEE in terms of recovery of antioxidant enzymes expression, down-regulation of eIF-2α and p38 phosphorylation, and inhibition of cell death are associated with reduced oxidative stress in SH-SY5Y cells.