압박성 궤양 치료를 위한 신선태을고의 효능 및 기전 연구

• Published in: 대한본초학회지(본초분과학회지) Vol. 40; no. 1; pp. 1 - 12
• Main Authors: 신미래, Mi-rae Shin, 이태종, Tae Jong Lee, 서정복, Jeong Bok Seo, 김민주, Min Ju Kim, 정일하, Il-ha Jeong, 안희연, Hui Yeon An, 노성수, Seong-soo Roh
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 31.01.2025
• Subjects: Ischemia-reperfusion injury; Pressure ulcers; Shenxian Taiyi Ointment; Wound healing; 한의학
• ISSN: 1229-1765; 2288-7199

Objectives : This study evaluated the wound-healing effect and mechanism of Shenxian Taiyi Ointment (STO) in a murine pressure ulcers (PU) model induced by ischemia/reperfusion (I/R) injury. Methods : The PU mouse model was developed through repeated cycles of I/R-induced pressure injury, followed by a daily application of STO for 12 days. Serum levels of reactive oxygen species (ROS), malondialdehyde (MDA), and inflammatory cytokines were measured. Western blotting was performed to assess nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase, inflammatory pathways, cell adhesion molecules, and proteins involved in extracellular matrix (ECM) degradation. Histopathological changes were investigated using Hematoxylin & Eosin (H&E) staining. Results : STO significantly reduced wound area and inhibited serum ROS and MDA levels by 35.8% and 53.5%, respectively. It also suppressed serum tumor necrosis factor (TNF)-α and interleukin (IL-6) by 25.3% and 51.9%, respectively. Additionally, STO decreased the protein expression of NADPH oxidase, a key producer of ROS, and significantly downregulated inflammatory enzymes, cytokines, and cell adhesion molecules by modulating nuclear factor kappa B (NF-κB). Moreover, STO decreased the expression of matrix metalloproteinases (MMP-1 and -13). The H&E staining results showed that STO improved histological changes caused by PU. Conclusions : STO holds enormous therapeutic potential for wound healing and the treatment of PU through the inhibition of ROS and the regulation of inflammatory mechanism.