류마티스 관절염 환자의 활액과 활막조직에서의 IL - 6 의 활성도

• Published in: The Korean journal of medicine Vol. 45; no. 2; pp. 235 - 243
• Main Authors: 조석구, Seok Goo CHo, 이상헌, Sang Heon Lee, 홍연식, Yeon Sik Hong, 조철수, Chul Soo Cho, 박석영, Seok Young Park, 박동준, Dong Jun Park, 김호연, Ho Youn Kim, 이정용, Jung Young Lee, 김상호, Sang Ho Kim, 변광호, Kwang Ho Pyun
• Format: Journal Article
• Language: Korean
• Published: 대한내과학회 01.08.1993
• Subjects: Interleukin - 6 ( IL - 6 ); Rheumatoid arthritis ( RA )
• ISSN: 1738-9364

Background: Interleukin-6 (IL-6), previously known as B cell stimulatory factor 2 (BSF-2) is a multifunctional cytokine that is produced by a various cells and plays an important role in the host defence mechanism such as regulation of immune response, acute phase reaction and hematopoiesis. Recent investigations have demonstrated that unregulated expression of IL-6 gene and overproducion of lL-6 were involved in the pathogenesis of cardiac myxoma, Castleman's disease, autoimmune disease, proliferative glomerulonephritis and certain lymphoid malignancies, especially plasmacytoma/multiple myelomas. We tried to evaluate the regulatory effect of IL 6 an the pathogenesis of rheumatoid arthritis (RA) which is characterized by hyperactivation of 8 cells, presence of various autoantibodies and increase in acute phase proteins and platelets. Methods: The IL-6 activity was measured by bioassay using the murine IL-6 dependent hybridoma cell lines (MH60. BSF2), ELISA method and immunohistochemical analysis. Results: 1) IL-6 activity was significantly elevated m synovial fluid from patients with rheumatoid arthritis (RA, n= 25) as compared with osteoarthrits (OA, n=6) and seronegative arthritis (SNA, n=4) (p<0.05). 2) IL-6 activity was also elevated in serum from patients with RA as compared with other disease (P <0.01). 3) High IL-6 activity and destructive changes in affected joints had a tendency to be correlated (r=0.716), And positive correlations between IL-6 activity and C-reactive proteins in serum were observed in patients with RA (r=0.752). 4) Immunohistachemical analysis demonstrated that positive-staining cells were located on the perivascular space. Conclusion: The results suggest that unregulated production of IL-6 may play an important role in the pathogenesis of RA and could explain local as well as generalized symptoms of RA. However, it is not known whether excessive production of IL-6 is a primary event in the disease process, or secondary consequence.