아임계수 추출 기술을 이용한 당귀 추출물의 유효성분 및 산화방지 평가

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 49; no. 1; pp. 44 - 49
• Main Authors: 고민정(Min-Jung Ko), 이정현(Jeong-Hyun Lee), 남화현(Hwa-Hyun Nam), 정명수(Myong-Soo Chung)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.02.2017; Korean Society of Food Science & Technology
• Subjects: Angelica gigas; Antioxidants; Dielectric constant; Equivalence; Flavonoids; Gallic acid; High pressure; High-performance liquid chromatography; Liquid chromatography; Phenolic compounds; Phenols; Quercetin; Scavenging; 식품과학; decursin; nodakenin; Angelica gigas Nakai; subcritical water extraction; antioxidant activity
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2017.49.1.44

Subcritical water extraction can be used to selectively extraction compounds by varying the temperaturedependent dielectric constant of water. This study investigated subcritical water extraction of decursin and nodakenin yields from Angelica gigas Nakai (AN) quantitatively and qualitatively by HPLC, and HPLC-ESI/MS. Total phenolics, total flavonoid contents, and antioxidant activity were determined by DPPH and ABTS radical scavenging activity, including the effects of varying the extraction conditions of temperature (110-200°C) and time (1-20 min) under high pressure (10 MPa). By subcritical water extraction under operating conditions of 120-130°C, the maximum yields of decursin (6.64±0.42% in the dried material) and nodakenin (3.71±0.28% in the dried material) were obtained. From 190-200°C the maximum yields of total phenolics (75.97±1.64 mg gallic acid equivalent/g AN), flavonoids (8.56±1.10 mg quercetin equivalent/g AN), DPPH (63.07±1.71%), and ABTS (72.32±2.82%) were obtained.